Short-term specialized enteral diet fails to attenuate malnutrition impairment of experimental open wound acute healing

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dc.contributor.authorAlves, Claudia Cristina [UNIFESP]
dc.contributor.authorTorrinhas, Raquel Susana
dc.contributor.authorGiorgi, Ricardo
dc.contributor.authorBrentani, Maria Mitzi
dc.contributor.authorLogullo, Angela Flavia [UNIFESP]
dc.contributor.authorArias, Victor [UNIFESP]
dc.contributor.authorMauad, Thais
dc.contributor.authorFerraz da Silva, Luiz Fernando
dc.contributor.authorWaitzberg, Dan L.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:05:25Z
dc.date.available2016-01-24T14:05:25Z
dc.date.issued2010-09-01
dc.description.abstractObjective: We assessed the effect of enteral refeeding on the morphology, gene expression, and contraction of acute open wounds in previously malnourished rats using two different enteral diets.Methods: Adult male isogenic Lewis rats divided into two groups (eutrophic, n = 30; and previously malnourished, 12-15% body weight loss, n = 27) were subjected to cutaneous dorsal wounds and gastrostomy. Control rats received a standard oral diet (AIN-93M chow) plus enteral saline solution. Subject rats received chow plus a standard enteral diet or an enteral diet enriched with arginine and antioxidants. On post-trauma days 7 and 14, wound granulation tissue samples were collected for morphologic analysis using hematoxylin and eosin and picrosirius stain or immunohistochemistry slides and real-time polymerase chain reaction for collagen I and III gene expression. Wound contraction was also evaluated by comparing wound images from days 0,7, and 14.Results: Malnourished control rats had increased intensity and duration of wound inflammation, impaired increase of fibroblast cells contingent on post-trauma days 7 to 14, decreased expression of collagen III, and less wound contraction compared with eutrophic control rats. A specialized enteral diet did not improve wound healing of malnourished rats but did promote wound contraction at post-trauma day 7 in eutrophic rats.Conclusion: Short-term enteral refeeding, even with a specialized diet, failed to protect previously wounded malnourished rats from a prolonged inflammatory phase and impaired healing. (C) 2010 Elsevier Inc. All rights reserved.en
dc.description.affiliationUniv São Paulo, Sch Med, Dept Gastroenterol,Metanutri Team, Lab Nutr & Metab Surg Digest Syst LIM 35, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Hlth Sci, Santos, Brazil
dc.description.affiliationUniv São Paulo, Sch Med, Dept Internal Med,Endocrinol Discipline, Human Nutr & Metab Dis Lab LIM 25, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Sch Med, Dept Radiol, Expt Oncol Dept LIM 24, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Pathol, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Sch Med, Dept Pathol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Hlth Sci, Santos, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Pathol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 05/54185-5
dc.format.extent873-879
dc.identifierhttp://dx.doi.org/10.1016/j.nut.2010.05.003
dc.identifier.citationNutrition. New York: Elsevier B.V., v. 26, n. 9, p. 873-879, 2010.
dc.identifier.doi10.1016/j.nut.2010.05.003
dc.identifier.issn0899-9007
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/32880
dc.identifier.wosWOS:000280925200007
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofNutrition
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectWound healingen
dc.subjectMalnutritionen
dc.subjectArginineen
dc.subjectAntioxidantsen
dc.subjectGene expressionen
dc.subjectCollagenen
dc.subjectMacrophagesen
dc.titleShort-term specialized enteral diet fails to attenuate malnutrition impairment of experimental open wound acute healingen
dc.typeinfo:eu-repo/semantics/article
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