Molecular characterization and immunolocalization of Schistosoma mansoni ATP-diphosphohydrolase

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dc.contributor.authorDeMarco, R.
dc.contributor.authorKowaltowski, A. T.
dc.contributor.authorMortara, R. A.
dc.contributor.authorVerjovski-Almeida, S.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:33:59Z
dc.date.available2016-01-24T12:33:59Z
dc.date.issued2003-08-08
dc.description.abstractSchistosoma mansoni, a human parasite that constitutes a major health problem in developing countries, escapes from host defenses and survives in the human bloodstream. Here, we report the cloning of a S. mansoni ATP-diphosphohydrolase ortholog (SmATPDase1). Southern blots indicated that in S. mansoni it is a single-copy gene. RT-PCR revealed that SmATPDase1 is expressed in five stages of the parasite life cycle, namely cercaria, schistosomula, adults, eggs, and miracidia. Using confocal microscopy, SmATPDase1 protein was immunolocalized on the external surface in all stages, except eggs, being conspicuously present in adults. ATPDase, which is present on the outer surface of endothelial cells lining human blood vessels, has been implicated in thromboregulation by promoting ADP hydrolysis and inhibition of platelet aggregation. the presence of an ATPDase ortholog on the surface of S. mansoni suggests that the enzyme might play a role in the escape from host defenses that would involve platelet activation. (C) 2003 Elsevier Inc. All rights reserved.en
dc.description.affiliationUniv São Paulo, Inst Quim, Dept Bioquim, BR-05508900 São Paulo, Brazil
dc.description.affiliationUNIFESP, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, São Paulo, Brazil
dc.description.affiliationUnifespUNIFESP, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent831-838
dc.identifierhttp://dx.doi.org/10.1016/S0006-291X(03)01268-3
dc.identifier.citationBiochemical and Biophysical Research Communications. San Diego: Academic Press Inc Elsevier Science, v. 307, n. 4, p. 831-838, 2003.
dc.identifier.doi10.1016/S0006-291X(03)01268-3
dc.identifier.issn0006-291X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/27362
dc.identifier.wosWOS:000184510100013
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofBiochemical and Biophysical Research Communications
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectSchistosoma mansonien
dc.subjectATP-diphosphohydrolaseen
dc.subjectATPDaseen
dc.subjectcloningen
dc.subjectimmunolocalizationen
dc.subjectparasite escapeen
dc.titleMolecular characterization and immunolocalization of Schistosoma mansoni ATP-diphosphohydrolaseen
dc.typeinfo:eu-repo/semantics/article
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