Histopathological, cytotoxicity and genotoxicity evaluation of Biosilicate (R) glass-ceramic scaffolds

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dc.contributor.authorKido, Hueliton Wilian
dc.contributor.authorOliveira, Poliani de
dc.contributor.authorParizotto, Nivaldo Antonio
dc.contributor.authorCrovace, Murilo Camuri
dc.contributor.authorZanotto, Edgar Dutra
dc.contributor.authorPeitl-Filho, Oscar
dc.contributor.authorFernandes, Kristianne Porta Santos
dc.contributor.authorMesquita Ferrari, Raquel Agnelli
dc.contributor.authorRibeiro, Daniel Araki [UNIFESP]
dc.contributor.authorRenno, Ana Claudia Muniz [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.contributor.institutionNove de Julho Univ UNINOVE
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:31:19Z
dc.date.available2016-01-24T14:31:19Z
dc.date.issued2013-03-01
dc.description.abstractThis study evaluated the biocompatibility of Biosilicate (R) scaffolds by means of histopathological, cytotoxicity, and genotoxicity analysis. the histopathologic analysis of the biomaterial was performed using 65 male rats, distributed into the groups: control and Biosilicate (R), evaluated at 7, 15, 30, 45, and 60 days after implantation. the cytotoxicity analysis was performed by the methyl thiazolyl tetrazolium (MTT) assay, with various concentrations of extracts from the biomaterial in culture of osteoblasts and fibroblasts after 24, 72, and 120 h. the genotoxicity analysis (comet assay) was performed in osteoblasts and fibroblasts after contact with the biomaterial during 24, 72, and 96 h. in the histopathology analysis, we observed a foreign body reaction, characterized by the presence of granulation tissue after 7 days of implantation of the biomaterial, and fibrosis connective tissue and multinucleated giant cells for longer periods. in the cytotoxicity analysis, extracts from the biomaterial did not inhibit the proliferation of osteoblasts and fibroblasts, and relatively low concentrations (12.5% and 25%) stimulated the proliferation of both cell types after 72 and 120 h. the analysis of genotoxicity showed that Biosilicate (R) did not induce DNA damage in both lineages tested in all periods. the results showed that the Biosilicate (R) scaffolds present in vivo and in vitro biocompatibility. (C) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 667673, 2013.en
dc.description.affiliationFed Univ Sao Carlos UFSCar, Postgrad Program Biotechnol, Dept Physiotherapy, Sao Carlos, SP, Brazil
dc.description.affiliationFed Univ Sao Carlos UFSCar, Vitreous Mat Lab LaMaV, Dept Mat Engn, Sao Carlos, SP, Brazil
dc.description.affiliationNove de Julho Univ UNINOVE, Dept Rehabil Sci & Biophoton Appl Hlth Sci, São Paulo, Brazil
dc.description.affiliationFed Univ São Paulo UNIFESP, Dept Biosci, Santos, SP, Brazil
dc.description.affiliationUnifespFed Univ São Paulo UNIFESP, Dept Biosci, Santos, SP, Brazil
dc.description.sourceWeb of Science
dc.format.extent667-673
dc.identifierhttps://dx.doi.org/10.1002/jbm.a.34360
dc.identifier.citationJournal of Biomedical Materials Research Part A. Hoboken: Wiley-Blackwell, v. 101, n. 3, p. 667-673, 2013.
dc.identifier.doi10.1002/jbm.a.34360
dc.identifier.issn1549-3296
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/36021
dc.identifier.wosWOS:000314112500007
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofJournal of Biomedical Materials Research Part A
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.subjectBiocompatibilityen
dc.subjectTissue responseen
dc.subjectBioactive materialen
dc.subjectScaffolden
dc.subjectBiomaterialen
dc.titleHistopathological, cytotoxicity and genotoxicity evaluation of Biosilicate (R) glass-ceramic scaffoldsen
dc.typeinfo:eu-repo/semantics/article
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