Cytokines and microbicidal molecules regulated by IL-32 in THP-1-derived human macrophages infected with New World Leishmania species

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dc.citation.issue2
dc.citation.volume11
dc.contributor.authordos Santos, Jessica Cristina
dc.contributor.authorHeinhuis, Bas
dc.contributor.authorGomes, Rodrigo Saar
dc.contributor.authorDamen, Michelle S. M. A.
dc.contributor.authorReal, Fernando [UNIFESP]
dc.contributor.authorMortara, Renato A. [UNIFESP]
dc.contributor.authorKeating, Samuel T.
dc.contributor.authorDinarello, Charles A.
dc.contributor.authorJoosten, Leo A. B.
dc.contributor.authorRibeiro-Dias, Fatima
dc.coverageSan Francisco
dc.date.accessioned2020-07-17T14:03:09Z
dc.date.available2020-07-17T14:03:09Z
dc.date.issued2017
dc.description.abstractBackground Interleukin-32 (IL-32) is expressed in lesions of patients with American Tegumentary Leishmaniasis (ATL), but its precise role in the disease remains unknown. Methodology/Principal findings In the present study, silencing and overexpression of IL-32 was performed in THP-1-derived macrophages infected with Leishmania (Viannia) braziliensis or L. (Leishmania) amazonensis to investigate the role of IL-32 in infection. We report that Leishmania species induces IL-32 gamma, and show that intracellular IL-32. protein production is dependent on endogenous TNF alpha. Silencing or overexpression of IL-32 demonstrated that this cytokine is closely related to TNF alpha and IL-8. Remarkably, the infection index was augmented in the absence of IL-32 and decreased in cells overexpressing this cytokine. Mechanistically, these effects can be explained by nitric oxide cathelicidin and beta-defensin 2 production regulated by IL-32. Conclusions Thus, endogenous IL-32 is a crucial cytokine involved in the host defense against Leishmania parasites.en
dc.description.affiliationRadboud Univ Nijmegen, Med Ctr, Dept Internal Med, Nijmegen, Netherlands
dc.description.affiliationRadboud Univ Nijmegen, Med Ctr, Radboud Ctr Infect Dis RCI, Nijmegen, Netherlands
dc.description.affiliationUniv Fed Goias, Inst Patol Trop & Saude Publ, Goiania, Go, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, Sao Paulo, Brazil
dc.description.affiliationUniv Colorado Denver, Div Infect Dis, Sch Med, Aurora, CO USA
dc.description.affiliationUnifespUniv Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
dc.description.sponsorshipCAPES
dc.description.sponsorshipDutch Arthritis Foundation
dc.description.sponsorshipIDCAPES: 401887/2013-8
dc.description.sponsorshipIDDutch Arthritis Foundation: 13-3-302
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1371/journal.pntd.0005413
dc.identifier.citationPlos Neglected Tropical Diseases. San Francisco, v. 11, n. 2, p. -, 2017.
dc.identifier.doi10.1371/journal.pntd.0005413
dc.identifier.fileWOS000396406600031.pdf
dc.identifier.issn1935-2735
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/55193
dc.identifier.wosWOS:000396406600031
dc.language.isoeng
dc.publisherPublic Library Science
dc.relation.ispartofPlos Neglected Tropical Diseases
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleCytokines and microbicidal molecules regulated by IL-32 in THP-1-derived human macrophages infected with New World Leishmania speciesen
dc.typeinfo:eu-repo/semantics/article
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