Synthesis, characterization and cytotoxicity of S-nitrosomercaptosuccinic acid-containing alginate/chitosan nanoparticles

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dc.citation.volume838
dc.contributor.authorSeabra, Amedea B. [UNIFESP]
dc.contributor.authorFabbri, Giulia K. [UNIFESP]
dc.contributor.authorPelegrino, Milena T.{UNIFESP]
dc.contributor.authorSilva, Leticia C.
dc.contributor.authorRodrigues, Tiago
dc.coverageBristol
dc.date.accessioned2020-07-17T14:03:32Z
dc.date.available2020-07-17T14:03:32Z
dc.date.issued2017
dc.description.abstractNitric oxide (NO) is an endogenous free radical, which plays key roles in several biological processes including vasodilation, neurotransmission, inhibition of platelet adhesion, cytotoxicity against pathogens, wound healing, and defense against cancer. Due to the relative instability of NO in vivo (half-life of ca. 0.5 seconds), there is an increasing interest in the development of low molecular weight NO donors, such as S-nitrosothiols (RSNOs), which are able to prolong and preserve the biological activities of NO in vivo. In order to enhance the sustained NO release in several biomedical applications, RSNOs have been successfully allied to nanomaterials. In this context, this work describes the synthesis and characterization of the NO donor S-nitroso-mercaptosuccinic acid (S-nitroso-MSA), which belongs to the class of RSNOs, and its incorporation in polymeric biodegradable nanoparticles composed by alginate/chitosan. First, chitosan nanoparticles were obtained by gelation process with sodium tripolyphosphate (TPP), followed by the addition of the alginate layer, to enhance the nanoparticle protection. The obtained nanoparticles presented a hydrodynamic diameter of 343 +/- 38 nm, polydispersity index (PDI) of 0.36 +/- 0.1, and zeta potential of -30.3 +/- 0.4 mV, indicating their thermal stability in aqueous suspension. The negative zeta potential value was assigned to the presence of alginate chains on the surface of chitosan/TPP nanoparticles. The encapsulation efficiency of the NO donor into the polymeric nanoparticles was found to be 98 +/- 0.2%. The high encapsulation efficiency value was attributed to the positive interactions between the NO donor and the polymeric content of the nanoparticles. Kinetics of NO release from the nanoparticles revealed a spontaneous and sustained release of therapeutic amounts of NO, for several hours under physiological temperature. The incubation of NO-releasing alginate/chitosan nanoparticles with human hepatocellular carcinoma (HepG2) cell line revealed a concentration-dependent toxicity. These results point to the promising uses of NO-releasing alginate/chitosan nanoparticles for anti-cancer chemotherapy.en
dc.description.affiliationUniv Fed ABC, Ctr Nat & Human Sci, Av Estados 5001, BR-09210580 Santo Andre, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Exact & Earth Sci Dept, Rua Sao Nicolau 210, BR-09913030 Diadema, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Exact & Earth Sci Dept, Rua Sao Nicolau 210, BR-09913030 Diadema, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipBrazilian Network on Nanotoxicology
dc.description.sponsorshipLaboratory of Nanostructure Synthesis and Biosystem Interactions-NANOBIOSS (MCTI)
dc.description.sponsorshipNewton Advanced Fellowship (The Royal Society)
dc.description.sponsorshipIDBrazilian Network on Nanotoxicology: 52120/2011-1
dc.description.sponsorshipIDLaboratory of Nanostructure Synthesis and Biosystem Interactions-NANOBIOSS (MCTI): 402280-2013
dc.description.sponsorshipIDNewton Advanced Fellowship (The Royal Society): NA140046
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1088/1742-6596/838/1/012032
dc.identifier.citation5th Nanosafe International Conference On Health And Safety Issues Related To Nanomaterials For A Socially Responsible Approach (Nanosafe 2016). Bristol, v. 838, p. -, 2017.
dc.identifier.doi10.1088/1742-6596/838/1/012032
dc.identifier.fileWOS000409540400032.pdf
dc.identifier.issn1742-6588
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/55471
dc.identifier.wosWOS:000409540400032
dc.language.isoeng
dc.publisherIop Publishing Ltd
dc.relation.ispartof5th Nanosafe International Conference on Health and Safety Issues Related to Nanomaterials for a Socially Responsible Approach (NANOSAFE)
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleSynthesis, characterization and cytotoxicity of S-nitrosomercaptosuccinic acid-containing alginate/chitosan nanoparticlesen
dc.typeinfo:eu-repo/semantics/conferenceObject
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