Decreased expression of proteins involved in energy metabolism in the hippocampal granular layer of rats submitted to the pilocarpine epilepsy model

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dc.contributor.authorAraujo, Bruno Henrique Silva [UNIFESP]
dc.contributor.authorTorres, Laila Brito [UNIFESP]
dc.contributor.authorDe Lima-Stein, Mariana Leão [UNIFESP]
dc.contributor.authorCabral, Francisco Romero
dc.contributor.authorHerai, Roberto Hirochi
dc.contributor.authorOkamoto, Oswaldo Keith
dc.contributor.authorCavalheiro, Esper Abrão [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionHosp Israelita Albert Einstein
dc.contributor.institutionFac Ciencias Med Santa Casa São Paulo
dc.contributor.institutionUniv Calif San Diego
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T14:35:19Z
dc.date.available2016-01-24T14:35:19Z
dc.date.issued2014-02-21
dc.description.abstractLong-term structural and functional changes in the hippocampus have been identified as the primary physiopathological basis for temporal lobe epilepsy. These changes include reactive gliosis and granule cell axonal sprouting within the dentate gyrus. the intimate mechanisms of these changes are beginning to be revealed. Here, we show the possibility of using laser capture microdissection (LCM) to isolate the dentate granular cell layer of Wistar rats submitted to the pilocarpine model of epilepsy. Using two-dimensional gel electrophoresis (2-D PAGE) and mass spectrometry for laser-captured cells, we identified molecular events that could be altered as part of the epileptic pathogenic process. According to our results, eight proteins related to energy metabolism were differentially expressed between both the control and pilocarpine-treated animals. These results provide, for the first time, new molecular insights into the altered protein profile of the epileptogenic dentate gyrus and can contribute to a better understanding of the phenomena involved in the genesis and maintenance of the epileptic state. (C) 2013 Elsevier Ireland Ltd. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Neurol Expt Escola Paulista Med, BR-04023900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Lab Biol Mol Parasitas, BR-04023900 São Paulo, Brazil
dc.description.affiliationHosp Israelita Albert Einstein, Inst Cerebra, São Paulo, Brazil
dc.description.affiliationFac Ciencias Med Santa Casa São Paulo, São Paulo, Brazil
dc.description.affiliationUniv Calif San Diego, Dept Pediat, Dept Cellular & Mol Med, Childrens Hosp San Diego,Sch Med,Stem Cell Progra, La Jolla, CA 92093 USA
dc.description.affiliationUniv São Paulo, Inst Biociencias, Dept Genet & Biol Evolut, BR-09500900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Neurol Expt Escola Paulista Med, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Lab Biol Mol Parasitas, BR-04023900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt
dc.description.sponsorshipCInAPCe (Brazil)
dc.description.sponsorshipIDFAPESP: 09/05764-3pt
dc.format.extent46-51
dc.identifierhttps://dx.doi.org/10.1016/j.neulet.2013.12.040
dc.identifier.citationNeuroscience Letters. Clare: Elsevier B.V., v. 561, p. 46-51, 2014.
dc.identifier.doi10.1016/j.neulet.2013.12.040
dc.identifier.issn0304-3940
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/37444
dc.identifier.wosWOS:000331858100009
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofNeuroscience Letters
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectEnergy metabolismen
dc.subjectTemporal lobe epilepsyen
dc.subjectLaser capture microdissectionen
dc.subjectTwo-dimensional gel electrophoresisen
dc.titleDecreased expression of proteins involved in energy metabolism in the hippocampal granular layer of rats submitted to the pilocarpine epilepsy modelen
dc.typeinfo:eu-repo/semantics/article
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