Disruption of the kinin B-1 receptor gene affects potentiating effect of captopril on BK-induced contraction in mice stomach fundus

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dc.contributor.authorBarbosa, Ana M. R. B.
dc.contributor.authorFelipe, Sandra A.
dc.contributor.authorPesquero, Joao B.
dc.contributor.authorPaiva, Antonio C. M.
dc.contributor.authorShimuta, Suma I.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:41:39Z
dc.date.available2016-01-24T12:41:39Z
dc.date.issued2006-12-01
dc.description.abstractA transgenic mouse model, deficient in kinin B-1 receptor (B-1(-/-)) was used to evaluate the role of B-2 receptor in the smooth muscle stomach fundus. the results showed that the potency of bradykinin (BK) to induce contraction in the gastric tissue was maintained whereas the efficacy was markedly reduced. the angiotensin converting enzyme (ACE) inhibitor captopril potentiated BK-induced effect in wild type (WT) but not in B-1(-/-) fundus. However, ACE activity detected by the convertion of Ang I to Ang II was inhibited by captopril in both types of gastric tissues. Taking into account the hypothesis that captopril and ACE bind to the B2 receptor, we suggest that this complex was not formed in the stomach deficient in B-1 receptor. Therefore, our finding strongly support the hypothesis that in smooth muscles that constitutively express the kinin B1 and B2 receptors, an interaction between captopril and ACE, B1 and B2 receptors should occur forming a complex protein interaction for the potentiating effect of ACE on kinin receptors. (c) 2006 Elsevier Inc. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Biophys, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Biophys, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent3377-3382
dc.identifierhttp://dx.doi.org/10.1016/j.peptides.2006.09.010
dc.identifier.citationPeptides. New York: Elsevier B.V., v. 27, n. 12, p. 3377-3382, 2006.
dc.identifier.doi10.1016/j.peptides.2006.09.010
dc.identifier.issn0196-9781
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/29300
dc.identifier.wosWOS:000242821300044
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofPeptides
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectkinin B-1 receptor knockouten
dc.subjectbradykininen
dc.subjectangiotensin converting enzymeen
dc.subjectinhibitoren
dc.titleDisruption of the kinin B-1 receptor gene affects potentiating effect of captopril on BK-induced contraction in mice stomach fundusen
dc.typeinfo:eu-repo/semantics/article
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