IgG purification by negative chromatography in amine-based ligands: A comparison of L-lysine and poly-L-lysine

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dc.contributor.authorLazzarotto Bresolin, Igor Tadeu [UNIFESP]
dc.contributor.authorFioritti, Renato Rodrigues
dc.contributor.authorAlves Buenoa, Sonia Maria
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:17:32Z
dc.date.available2016-01-24T14:17:32Z
dc.date.issued2011-12-01
dc.description.abstractAgarose gels with immobilized amine-based ligands L-lysine (Lys) and poly-L-lysine (PLL) exhibited the ability to adsorb human serum proteins and to separate immunoglobulin G (IgG) in nonretained chromatographic fractions (negative chromatography). the effect of the buffer system and pH on IgG purification on Lys-agarose and PLL-agarose showed that PLL was the most selective ligand. PLL-agarose was able to purify IgG from human serum solution diluted in Bis-Tris buffer at pH 6.0 with 79% yield and 88.7% purity (based on total protein concentration and nephelometric analysis of albumin, transferrin, and immunoglobulins A, G, and M). Human serum albumin (HSA) and IgG adsorption data showed that the first followed the Langmuir model whereas the second followed the Langmuir-Freundlich model, due to the presence of a positive cooperativity effect (n = 1.60), with the maximum adsorption capacities of 76.4 mg mL(-1) for HSA and 23.6 mg mL(-1) for IgG. the Delta G(max) values obtained by non-linear regression of HSA and IgG adsorption data of Temkin model were similar (-7.0 kcal mol(-1)), indicating that the process is spontaneous in nature. the joint analysis of these data shows that PLL-agarose can be considered an alternative adsorbent for the isolation of IgG from human serum with the potential to be integrated to a purification process on a large scale. (C) 2011 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv Estadual Campinas, Sch Chem Engn, UNICAMP, BR-13083852 Campinas, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, UNIFESP, Dept Exact & Earth Sci, BR-09972270 Diadema, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, UNIFESP, Dept Exact & Earth Sci, BR-09972270 Diadema, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent2277-2285
dc.identifierhttp://dx.doi.org/10.1016/j.procbio.2011.09.005
dc.identifier.citationProcess Biochemistry. Oxford: Elsevier B.V., v. 46, n. 12, p. 2277-2285, 2011.
dc.identifier.doi10.1016/j.procbio.2011.09.005
dc.identifier.issn1359-5113
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/34303
dc.identifier.wosWOS:000297832000009
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofProcess Biochemistry
dc.rightsAcesso restrito
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectIgG purificationen
dc.subjectHuman serumen
dc.subjectLysineen
dc.subjectPoly-L-lysineen
dc.subjectNegative chromatographyen
dc.subjectElectrostatic interactionsen
dc.titleIgG purification by negative chromatography in amine-based ligands: A comparison of L-lysine and poly-L-lysineen
dc.typeArtigo
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