A conserved dimorphism-regulating histidine kinase controls the dimorphic switching in Paracoccidioides brasiliensis

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dc.citation.issue5
dc.citation.volume16
dc.contributor.authorChaves, Alison F. A. [UNIFESP]
dc.contributor.authorNavarro, Marina V. [UNIFESP]
dc.contributor.authorCastilho, Daniele G. [UNIFESP]
dc.contributor.authorCalado, Juliana C. P. [UNIFESP]
dc.contributor.authorConceicao, Palloma M. [UNIFESP]
dc.contributor.authorBatista, Wagner L. [UNIFESP]
dc.coverageOxford
dc.date.accessioned2020-08-14T13:43:56Z
dc.date.available2020-08-14T13:43:56Z
dc.date.issued2016
dc.description.abstractParacoccidioides brasiliensis and P. lutzii, thermally dimorphic fungi, are the causative agents of paracoccidioidomycosis (PCM). Paracoccidioides infection occurs when conidia or mycelium fragments are inhaled by the host, which causes the Paracoccidioides cells to transition to the yeast form. The development of disease requires conidia inside the host alveoli to differentiate into yeast cells in a temperature-dependent manner. We describe the presence of a two-component signal transduction system in P. brasiliensis, which we investigated by expression analysis of a hypothetical protein gene (PADG_07579) that showed high similarity with the dimorphism-regulating histidine kinase (DRK1) gene of Blastomyces dermatitidis and Histoplasma capsulatum. This gene was sensitive to environmental redox changes, which was demonstrated by a dose-dependent decrease in transcript levels after peroxide stimulation and a subtler decrease in transcript levels after NO stimulation. Furthermore, the higher PbDRK1 levels after treatment with increasing NaCl concentrations suggest that this histidine kinase can play a role as osmosensing. In the mycelium-yeast (M -> Y) transition, PbDRK1 mRNA expression increased 14-fold after 24 h incubation at 37A degrees C, consistent with similar observations in other virulent fungi. These results demonstrate that the PbDRK1 gene is differentially expressed during the dimorphic M -> Y transition. Finally, when P. brasiliensis mycelium cells were exposed to a histidine kinase inhibitor and incubated at 37A degrees C, there was a delay in the dimorphic M -> Y transition, suggesting that histidine kinases could be targets of interest for PCM therapy.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Unidade Jose Alencar, St Sao Nicolau 210,4 Floor, BR-04023900 Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Ciencias Farmaceut, R Sao Nicolau 210, BR-09913030 Diadema, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Unidade Jose Alencar, St Sao Nicolau 210,4 Floor, BR-04023900 Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Ciencias Farmaceut, R Sao Nicolau 210, BR-09913030 Diadema, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/Brazil)
dc.description.sponsorshipFAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo/Brazil)
dc.description.sponsorshipIDCNPq: 478023/2013-8
dc.description.sponsorshipIDFAPESP: 2014/13961-1
dc.description.sponsorshipIDFAPESP: 2015/09727-6
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1093/femsyr/fow047
dc.identifier.citationFems Yeast Research. Oxford, v. 16, n. 5, p. -, 2016.
dc.identifier.doi10.1093/femsyr/fow047
dc.identifier.fileWOS000383231100005.pdf
dc.identifier.issn1567-1356
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/57446
dc.identifier.wosWOS:000383231100005
dc.language.isoeng
dc.publisherOxford Univ Press
dc.relation.ispartofFems Yeast Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectdimorphismen
dc.subjecthistidine kinaseen
dc.subjectphosphorelayen
dc.subjectParacoccidioidesen
dc.titleA conserved dimorphism-regulating histidine kinase controls the dimorphic switching in Paracoccidioides brasiliensisen
dc.typeinfo:eu-repo/semantics/article
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