Frequency of Chlamydia trachomatis infection in cervical intraepithelial lesions and the status of cytological p16/Ki-67 dual-staining

Página do item simplificado
dc.citation.volume12
dc.contributor.authorRobial, R.
dc.contributor.authorLongatto-Filho, A.
dc.contributor.authorRoteli-Martins, C. M.
dc.contributor.authorSilveira, M. F.
dc.contributor.authorStauffert, D.
dc.contributor.authorRibeiro, G. G.
dc.contributor.authorLinhares, I. M.
dc.contributor.authorTacla, M.
dc.contributor.authorZonta, M. A. [UNIFESP]
dc.contributor.authorBaracat, E. C.
dc.coverageLondon
dc.date.accessioned2020-07-17T14:03:16Z
dc.date.available2020-07-17T14:03:16Z
dc.date.issued2017
dc.description.abstractBackground: Chlamydia trachomatis (Ct) is not a disease subject to mandatory reporting in Brazil, and the prevalence rate of this genital infection varies according to the region in which studies are conducted, as well as by the detection technique employed. Ct has been associated with persistence of Human papillomavirus (HPV) infection and the facilitation of cervical carcinoma development. We evaluated the Chlamydia trachomatis infection and its association with cytology, p16/Ki-67 dual-stained cytology and cervical intraepithelial lesions status in a screening cohort in Brazil. Methods: This was a cross-sectional study of 1481 cervical samples from asymptomatic women aged 18 to 64. Samples were collected for liquid-based cytology and Ct detection by polymerase chain reaction. p16/Ki-67 double staining was performed on samples with abnormal cytology. Statistical analysis was by chi-square and likelihood-ratio tests. Odds ratio (OR) and 95% confidence intervals (95% CI) were determined. Results: The frequency of Ct was 15.6% and its presence was not associated with detection of p16/Ki-67 [OR = 1. 35 (0.5-3.4)]. There was also no association between abnormal cervical cytology and Ct-positivity [OR = 1.21 (0.46-3.2)]. Associations were observed between p16/Ki-67 and high-grade lesions detected by cytology and in biopsies [OR = 3.55 (1.50-8.42) and OR = 19.00 (0.6-7.2), respectively]. Conclusions: The asymptomatic women in our study had a high frequency of Ct infection but this was not associated with p16/Ki-67 detection in samples with abnormal cytology. The expression of p16/Ki-67 was highest in women with high-grade CIN (p = 0.003).en
dc.description.affiliationUniv Sao Paulo, Div Gynecol Clin, Fac Med, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Lab Med Invest LIM 14, Fac Med, Sao Paulo, Brazil
dc.description.affiliationPio XII Fdn, Barretos Canc Hosp, Mol Oncol Res Ctr, Barretos, Brazil
dc.description.affiliationUniv Minho, Life & Hlth Sci Res Inst ICVS, Sch Hlth Sci, Braga, Portugal
dc.description.affiliationICVS 3Bs PT Govt Associate Lab, Braga, Portugal
dc.description.affiliationSecretaria Saude Sao Paulo, Leonor M De Barros Hosp, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Pelotas, Pelotas, RS, Brazil
dc.description.affiliationUniv Ribeirao Preto, Sch Pharmaceut Sci, Ribeirao Preto, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Discipline Infectol, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Discipline Infectol, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1186/s13027-016-0111-8
dc.identifier.citationInfectious Agents And Cancer. London, v. 12, p. -, 2017.
dc.identifier.doi10.1186/s13027-016-0111-8
dc.identifier.fileWOS000391632700002.pdf
dc.identifier.issn1750-9378
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/55268
dc.identifier.wosWOS:000391632700002
dc.language.isoeng
dc.publisherBiomed Central Ltd
dc.relation.ispartofInfectious Agents And Cancer
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectChlamydiaen
dc.subjectCervical canceren
dc.subjectImmunocytochemistryen
dc.subjectki 67en
dc.titleFrequency of Chlamydia trachomatis infection in cervical intraepithelial lesions and the status of cytological p16/Ki-67 dual-stainingen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
WOS000391632700002.pdf
Tamanho:
470.18 KB
Formato:
Adobe Portable Document Format
Descrição:
Baixar
Coleções
EPM - Artigos