Peritoneal fluid modulates the sperm acrosomal exocytosis induced by N-acetylglucosaminyl neoglycoprotein

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dc.contributor.authorPassos, Eduardo Pandolfi
dc.contributor.authorBrugnara, L.
dc.contributor.authorFacin, A.C.
dc.contributor.authorRiffel, Alessandro
dc.contributor.authorLima, Geraldo Rodrigues de [UNIFESP]
dc.contributor.authorFreitas, Vilmon de [UNIFESP]
dc.contributor.authorBrandelli, Adriano
dc.contributor.institutionHospital de Clínicas de Porto Alegre
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2015-06-14T13:24:49Z
dc.date.available2015-06-14T13:24:49Z
dc.date.issued1999-01-01
dc.description.abstractThe effect of peritoneal fluid (PF) on the human sperm acrosome reaction (AR) was tested. Sperm was pre-incubated with PF and the AR was induced by calcium ionophore A23187 and a neoglycoprotein bearing N-acetylglycosamine residues (NGP). The AR induced by calcium ionophore was inhibited 40% by PF from controls (PFc) and 50% by PF from the endometriosis (PFe) group, but not by PF from infertile patients without endometriosis (PFi). No significant differences were found in the spontaneous AR. When the AR was induced by NGP, pre-incubation with PFc reduced (60%) the percentage of AR, while PFe and PFi caused no significant differences. The average rates of acrosome reactions obtained in control, NGP- and ionophore-treated sperm showed that NGP-induced exocytosis differed significantly between the PFc (11%) and PFe/PFi groups (17%), and the ionophore-induced AR was higher for PFi (33%) than PFc/PFe (25%). The incidence of the NGP-induced AR was reduced in the first hour of pre-incubation with PFc and remained nearly constant throughout 4 h of incubation. The present data indicate that PF possesses a protective factor which prevents premature AR.en
dc.description.affiliationHospital de Clínicas de Porto Alegre
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP)
dc.description.affiliationUnifespUNIFESP, EPM, São Paulo, Brazil
dc.description.sourceSciELO
dc.format.extent59-65
dc.identifierhttps://dx.doi.org/10.1590/S0100-879X1999000100009
dc.identifier.citationBrazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 32, n. 1, p. 59-65, 1999.
dc.identifier.doi10.1590/S0100-879X1999000100009
dc.identifier.fileS0100-879X1999000100009.pdf
dc.identifier.issn0100-879X
dc.identifier.scieloS0100-879X1999000100009
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/725
dc.identifier.wosWOS:000078324100009
dc.language.isoeng
dc.publisherAssociação Brasileira de Divulgação Científica
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAcrosome reactionen
dc.subjectEndometriosisen
dc.subjectInfertilityen
dc.subjectPeritoneal fluiden
dc.subjectSpermen
dc.titlePeritoneal fluid modulates the sperm acrosomal exocytosis induced by N-acetylglucosaminyl neoglycoproteinen
dc.typeinfo:eu-repo/semantics/article
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