Gold Nanoparticles Used as Protein Scavengers Enhance Surface Plasmon Resonance Signal

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dc.citation.issue12
dc.citation.volume17
dc.contributor.authorde Macedo, Erenildo Ferreira [UNIFESP]
dc.contributor.authorDucatti Formaggio, Daniela Maria [UNIFESP]
dc.contributor.authorSantos, Nivia Salles [UNIFESP]
dc.contributor.authorTada, Dayane Batista [UNIFESP]
dc.coverageBasel
dc.date.accessioned2020-07-02T18:52:21Z
dc.date.available2020-07-02T18:52:21Z
dc.date.issued2017
dc.description.abstractAlthough several researchers had reported on methodologies for surface plasmon resonance (SPR) signal amplification based on the use of nanoparticles (NPs), the majority addressed the sandwich technique and low protein concentration. In this work, a different approach for SPR signal enhancement based on the use of gold NPs was evaluated. The method was used in the detection of two lectins, peanut agglutinin (PNA) and concanavalin A (ConA). Gold NPs were functionalized with antibodies anti-PNA and anti-ConA, and these NPs were used as protein scavengers in a solution. After being incubated with solutions of PNA or ConA, the gold NPs coupled with the collected lectins were injected on the sensor containing the immobilized antibodies. The signal amplification provided by this method was compared to the signal amplification provided by the direct coupling of PNA and ConA to gold NPs. Furthermore, both methods, direct coupling and gold NPs as protein scavengers, were compared to the direct detection of PNA and ConA in solution. Compared to the analysis of free protein, the direct coupling of PNA and ConA to gold NPs resulted in a signal amplification of 10-40-fold and a 13-fold decrease of the limit of detection (LOD), whereas the use of gold NPs as protein scavengers resulted in an SPR signal 40-50-times higher and an LOD 64-times lower.en
dc.description.affiliationUniv Fed Sao Paulo, Inst Ciencia & Tecnol, Rua Talim 330, BR-12231280 Sao Jose Dos Campos, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Inst Ciencia & Tecnol, Rua Talim 330, BR-12231280 Sao Jose Dos Campos, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2011/23895-8, 2017/01697-6]
dc.description.sponsorshipIDFAPESP [2011/23895-8, 2017/01697-6]
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.3390/s17122765
dc.identifier.citationSensors. Basel, v. 17, n. 12, p. -, 2017.
dc.identifier.doi10.3390/s17122765
dc.identifier.fileWOS000423285800065.pdf
dc.identifier.issn1424-8220
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54023
dc.identifier.wosWOS:000423285800065
dc.language.isoeng
dc.publisherMdpi Ag
dc.relation.ispartofSensors
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectsurface plasmon resonanceen
dc.subjectgold nanoparticlesen
dc.subjectsignal amplificationen
dc.subjectlectinsen
dc.subjectconcanavalin Aen
dc.subjectpeanut agglutininen
dc.titleGold Nanoparticles Used as Protein Scavengers Enhance Surface Plasmon Resonance Signalen
dc.typeinfo:eu-repo/semantics/article
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