Mutational analysis of the interaction of the N- and C-terminal ends of angiotensin II with the rat AT(1A) receptor

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dc.contributor.authorCosta-Neto, Claudio M.
dc.contributor.authorMikakawa, Ayumi A.
dc.contributor.authorOliveira, Laerte
dc.contributor.authorHjorth, Siv A.
dc.contributor.authorSchwartz, Thue W.
dc.contributor.authorPaiva, Antonio Cechelli de Mattos [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Copenhagen
dc.date.accessioned2016-01-24T12:31:07Z
dc.date.available2016-01-24T12:31:07Z
dc.date.issued2000-07-01
dc.description.abstract1. the role of different residues of the rat AT(1A) receptor in the interaction with the N- and C-terminal ends of angiotensin II (AngII) was studied by determining ligand binding and production of inositol phosphates (IP) in COS-7 cells transiently expressing the following AT(1A) mutants: T88H, Y92H, G196I, G196W and D278E.2 G196W and G196I retained significant binding and IF-production properties, indicating that bulky substituents in position 196 did not affect the interaction of AngII's C-terminal carboxyl with Lys(199) located three residues below.3 Although the T88A mutation did not affect binding, the T88H mutant had greatly decreased affinity for AngII, suggesting that substitution of Thr(88) by His might hinder binding through an indirect effect.4 the Y92H mutation caused loss of affinity for AngII that was much less pronounced than that reported for Y92A, indicating that His in that position can fulfil part of the requirements for binding.5 Replacing Asp(278) by Glu caused a much smaller reduction in affinity than replacing it by Ala, indicating the importance of Asp's beta-carboxyl group for AngII binding.6 Mutations in residues Thr(88), Tyr(92) and Asp(278) greatly reduced affinity for AngII but not for Sar(1) Leu(8)-AngII, suggesting unfavourable interactions between these residues and AngII's aspartic acid side-chain or N-terminal amino group, which might account for the proposed role of the N-terminal amino group of AngII in the agonist-induced desensitization (tachyphylaxis) of smooth muscles.en
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Biophys, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniv Copenhagen, Panum Inst, Mol Pharmacol Lab, Copenhagen, Denmark
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Biophys, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent1263-1268
dc.identifierhttp://dx.doi.org/10.1038/sj.bjp.0703430
dc.identifier.citationBritish Journal of Pharmacology. Basingstoke: Nature Publishing Group, v. 130, n. 6, p. 1263-1268, 2000.
dc.identifier.doi10.1038/sj.bjp.0703430
dc.identifier.issn0007-1188
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/26332
dc.identifier.wosWOS:000088383500010
dc.language.isoeng
dc.publisherNature Publishing Group
dc.relation.ispartofBritish Journal of Pharmacology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectangiotensin IIen
dc.subjectAT(1) receptoren
dc.subjectG-proteinen
dc.subjectsite-directed mutagenesisen
dc.titleMutational analysis of the interaction of the N- and C-terminal ends of angiotensin II with the rat AT(1A) receptoren
dc.typeinfo:eu-repo/semantics/article
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