Lipases and lipid droplet-associated protein expression in subcutaneous white adipose tissue of cachectic patients with cancer

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dc.contributor.authorSilverio, Renata
dc.contributor.authorLira, Fabio Santos de
dc.contributor.authorOyama, Lila Missae [UNIFESP]
dc.contributor.authorNascimento, Claudia Maria da Penha Oller do [UNIFESP]
dc.contributor.authorOtoch, Jose Pinhata
dc.contributor.authorAlcantara, Paulo S. M.
dc.contributor.authorBatista Junior, Miguel Luiz
dc.contributor.authorSeelaender, Marilia
dc.date.accessioned2019-08-19T11:49:42Z
dc.date.available2019-08-19T11:49:42Z
dc.date.issued2017
dc.description.abstractBackground: Cancer cachexia is a multifactorial metabolic syndrome characterized by marked loss of adipose tissue and skeletal muscle. Fat loss from adipose tissue in cancer cachexia is partly the result of increased lipolysis. Despite the growing amount of studies focused on elucidating the mechanisms through which lipolysis-related proteins regulate the lipolytic process, there are scarce data concerning that profile in the adipose tissue of cancer cachectic patients. Considering its fundamental importance, it was our main purpose to characterize the expression of the lipolysis-related proteins in the white adipose tissue of cachectic cancer patients. Methods: Patients from the University Hospital were divided into three groups: control, cancer cachexia (CC), and weight-stable cancer patients (WSC). To gain greater insight into adipose tissue wasting during cancer cachexia progression, we have also analyzed an experimental model of cachexia (Walker 256 carcinosarcoma). Animals were divided into: control, intermediate cachexia (IC) and terminal cachexia (TC). Subcutaneous white adipose tissue of patients and epidydimal white adipose tissue of animals were investigated regarding molecular aspects by determining the protein content and gene expression of hormone-sensitive lipase (HSL), adipose triglyceride lipase (ATGL), comparative gene identification-58 (CGI-58), perilipin 1, leptin, adiponectin, visfatin, and tumour necrosis factor alpha (TNF-alpha). Results: We found augmented lipolysis in CC associated with increased HSL expression, as well as upregulation of ATGL expression and reduction in perilipin 1 content. In IC, there was an imbalance in the secretion of pro-and anti-inflammatory factors. The alterations at the end-stage of cachexia were even more profound, and there was a reduction in the expression of almost all proteins analyzed in the animals. Conclusions: Our findings show that cachexia induces important morphological, molecular, and humoral alterations in the white adipose tissue, which are specific to the stage of the syndrome.en
dc.description.affiliationUniv São Paulo, Canc Metab Res Grp, Inst Biomed Sci, Dept Surg,Fac Med, São Paulo, Brazil
dc.description.affiliationSão Paulo State Univ UNESP, Exercise & Immunometab Res Grp, Dept Phys Educ, Presidente Prudente, SP, Brazil
dc.description.affiliationUniv Fed São Paulo, UNIFESP, Dept Fisiol, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Univ Hosp, Dept Clin Surg, São Paulo, Brazil
dc.description.affiliationUniv Mogi das Cruzes, Lab Adipose Tissue Biol, Ctr Integrated Biotechnol, Mogi Das Cruzes, Brazil
dc.description.affiliationUniv São Paulo, Inst Biomed Sci, Ave Prof Lineu Prestes 1524,Lab 434, BR-05508900 São Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed São Paulo, UNIFESP, Dept Fisiol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt
dc.description.sponsorshipIDFAPESP: 2012/50079-0
dc.format.extent-
dc.identifierhttps://dx.doi.org/10.1186/s12944-017-0547-x
dc.identifier.citationLipids In Health And Disease. London, v. 16, p. -, 2017.
dc.identifier.doi10.1186/s12944-017-0547-x
dc.identifier.fileWOS000408372700002.pdf
dc.identifier.issn1476-511X
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/51379
dc.identifier.wosWOS:000408372700002
dc.language.isoeng
dc.publisherBiomed Central Ltd
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleLipases and lipid droplet-associated protein expression in subcutaneous white adipose tissue of cachectic patients with canceren
dc.typeinfo:eu-repo/semantics/article
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