Characterization and biological evaluation of the introduction of PLGA into biosilicate

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dc.citation.issue5]
dc.citation.volume105]
dc.contributor.authorFernandes, Kelly Rossetti [UNIFESP]
dc.contributor.authorMagri, Angela Maria Paiva [UNIFESP]
dc.contributor.authorKido, Hueliton Wilian [UNIFESP]
dc.contributor.authorUeno, Fabio Roberto [UNIFESP]
dc.contributor.authorAssis, Livia [UNIFESP]
dc.contributor.authorFernandes, Kristianne Porta Santos
dc.contributor.authorMesquita Ferrari, Raquel Agnelli
dc.contributor.authorMartins, V. C.
dc.contributor.authorPlepis, Ana M.
dc.contributor.authorZanotto, Edgar Dutra
dc.contributor.authorPeitl Filho, Oscar
dc.contributor.authorRibeiro, Daniel Araki [UNIFESP]
dc.contributor.authorVan den Beucken, Jeroen J.
dc.contributor.authorRenno, Ana Claudia Muniz [UNIFESP]
dc.coverageHoboken
dc.date.accessioned2020-06-26T16:30:30Z
dc.date.available2020-06-26T16:30:30Z
dc.date.issued2017
dc.description.abstractThe aims of this study were to characterize different BS/PLGA composites for their physicochemical and morphological characteristics and evaluate the in vitro and in vivo biological performance. The physicochemical and morphological modifications were analyzed by pH, mass loss, XRD, setting time, and SEM. For in vitro analysis, the osteoblast and fibroblast viability was evaluated. For in vivo evaluations, histopathology and immunohistochemistry were performed in a tibial defect in rats. After incubation, all composites presented lower values in pH and mass loss over time. Moreover, XRD and SEM analysis confirmed that the composites degraded over time. Additionally, pore formation was observed by SEM analysis after incubation mainly in BS/PLGA groups. BS/PLGA showed significantly increased in osteoblast viability 24 h. Moreover, BS/PLGA composites demonstrated an increase in fibroblast viability in all periods analyzed when compared to BS. In the in vivo study, after 2 and 6 weeks of implantation of biomaterials, histopathological findings revealed that the BS/PLGA composites degrades over time, mainly at periphery. Moreover, can be observed the presence of granulation tissue, bone formation, Runx-2, and RANKL immunoexpression in all groups. In conclusion, BS/PLGA composites present appropriate physicochemical characteristics, stimulate the cellular viability, and enhance the bone repair in vivo. (C) 2016 Wiley Periodicals, Inc.en
dc.description.affiliationFed Univ Sao Paulo UNIFESP, Dept Biosci, Santos, SP, Brazil
dc.description.affiliationNove de Julho Univ UNINOVE, Dept Rehabil Sci & Biophoton Appl Hlth Sci, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Inst Chem, Sao Carlos, SP, Brazil
dc.description.affiliationFed Univ Sao Carlos UFSCar, Dept Mat Engn, Vitreous Mat Lab LaMaV, Sao Carlos, SP, Brazil
dc.description.affiliationRadboudumc, Dept Biomat, Nijmegen, Netherlands
dc.description.affiliationUnifespFed Univ Sao Paulo UNIFESP, Dept Biosci, Santos, SP, Brazil
dc.description.sourceWeb of Science
dc.format.extent1063-1074
dc.identifierhttps://dx.doi.org/10.1002/jbm.b.33654]
dc.identifier.citationJournal Of Biomedical Materials Research Part B-Applied Biomaterials. Hoboken, v. 105, n. 5, p. 1063-1074, 2017.
dc.identifier.doi10.1002/jbm.b.33654
dc.identifier.issn1552-4973
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/53580
dc.identifier.wosWOS:000405311700013
dc.language.isoeng
dc.publisherWiley
dc.relation.ispartofJournal Of Biomedical Materials Research Part B-Applied Biomaterials
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectBiomaterials availabilityen
dc.subjectBioactive glassen
dc.subjectPolymeren
dc.subjectCytotoxicityen
dc.subjectBoneen
dc.titleCharacterization and biological evaluation of the introduction of PLGA into biosilicateen
dc.typeinfo:eu-repo/semantics/article
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