Juruin: an antifungal peptide from the venom of the Amazonian Pink Toe spider, Avicularia juruensis, which contains the inhibitory cystine knot motif

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dc.contributor.authorAyroza, Gabriela
dc.contributor.authorFerreira, Ivan L. C.
dc.contributor.authorSayegh, Raphael S. R.
dc.contributor.authorTashima, Alexandre Keiji [UNIFESP]
dc.contributor.authorSilva, Pedro I. da
dc.contributor.institutionInst Butantan
dc.contributor.institutionCoordenadoria Controle Doencas
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:17:39Z
dc.date.available2016-01-24T14:17:39Z
dc.date.issued2012-01-01
dc.description.abstractThe aim of this study was to screen the venom of the theraposid spider Avicularia juruensis for the identification of antimicrobial peptides (AMPs) which could be further used as prototypes for drug development. Eleven AMPs, named juruentoxins, with molecular weight ranging from 3.5 to 4.5 kDa, were identified by mass spectrometry after the soluble venom was separated by high performance liquid chromatography. Juruentoxins have a putative inhibitory cystine knot (ICK) motif, generally found in neurotoxins, which are also resistant to proteolysis. One juruentoxin that has 38 amino acid residues and three disulfide bonds were characterized, to which we proposed the name Juruin. Based on liquid growth inhibition assays, it has potent antifungal activity in the micromolar range. Importantly, Juruin lacks haemolytic activity on human erythrocytes at the antimicrobial concentrations. Based on the amino acid sequence, it is highly identical to the insecticidal peptides from the theraposid spiders Selenocosmia huwena, Chilobrachys jingzhao, and Haplopelma schmidti from China, indicating they belong to a group of conserved toxins which are likely to inhibit voltage-gated ion channels. Juruin is a cationic AMP and Lys22 and Lys23 show maximum positive charge localization that might be important for receptor recognition. Although it shows marked sequence similarity to neurotoxic peptides, Juruin is a novel exciting molecule with potent antifungal activity, which could be used as a novel template for development of drugs against clinical resistant fungi strains.en
dc.description.affiliationInst Butantan, Lab Especial Toxinol Aplicada, BR-05503900 São Paulo, Brazil
dc.description.affiliationCoordenadoria Controle Doencas, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Ciencias Exatas & Terra, Diadema, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Ciencias Exatas & Terra, Diadema, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent10
dc.identifierhttp://dx.doi.org/10.3389/fmicb.2012.00324
dc.identifier.citationFrontiers in Microbiology. Lausanne: Frontiers Research Foundation, v. 3, 10 p., 2012.
dc.identifier.doi10.3389/fmicb.2012.00324
dc.identifier.fileWOS000208863600328.pdf
dc.identifier.issn1664-302X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/34400
dc.identifier.wosWOS:000208863600328
dc.language.isoeng
dc.publisherFrontiers Research Foundation
dc.relation.ispartofFrontiers in Microbiology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectJuruinen
dc.subjectjuruentoxinsen
dc.subjectAvicularia juruensisen
dc.subjectinhibitory cystine knot motifen
dc.subjectTheraphosidae venomen
dc.subjectantimicrobial peptidesen
dc.titleJuruin: an antifungal peptide from the venom of the Amazonian Pink Toe spider, Avicularia juruensis, which contains the inhibitory cystine knot motifen
dc.typeinfo:eu-repo/semantics/article
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