Effects of drinking desalinated seawater on cell viability and proliferation

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dc.citation.issue3
dc.citation.volume15
dc.contributor.authorMacarrao, Camila Longhi
dc.contributor.authorLacerda Bachi, Andre Luis
dc.contributor.authorMariano, Mario [UNIFESP]
dc.contributor.authorAbel, Lucia Jamli
dc.coverageLondon
dc.date.accessioned2020-07-13T11:53:02Z
dc.date.available2020-07-13T11:53:02Z
dc.date.issued2017
dc.description.abstractDesalination of seawater is becoming an important means to address the increasing scarcity of freshwater resources in the world. Seawater has been used as drinking water in the health, food, and medical fields and various beneficial effects have been suggested, although not confirmed. Given the presence of 63 minerals and trace elements in drinking desalinated seawater (63 DSW), we evaluated their effects on the behavior of tumorigenic and nontumorigenic cells through the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay and annexin-V-fluorescein isothiocyanate/propidium iodide staining. Our results showed that cell viability and proliferation in the presence of 63 DSW were significantly greater than in mineral water and in the presence of fetal bovine serum in a dose-dependent manner. Furthermore, 63 DSW showed no toxic effect on murine embryonic fibroblast (NIH-3T3) and murine melanoma (B16-F10) cells. In another assay, we also showed that pre-treatment of non-adherent THP-1 cells with 63 DSW reduces apoptosis incidence, suggesting a protective effect against cell death. We conclude that cell viability and proliferation were improved by the mineral components of 63 DSW and this effect can guide further studies on health effects associated with DSW consumption.en
dc.description.affiliationUniv Paulista, UNIP, Av Dr Bacelar, Sao Paulo 1212, SP, Brazil
dc.description.affiliationUniv Cruzeiro Sul, Inst Ciencias Atividade Fis & Esporte, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, UNIFESP, Dept Imunol, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, UNIFESP, Dept Imunol, Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipUNIP
dc.description.sponsorshipUNIFESP
dc.description.sponsorshipPROSUP
dc.format.extent360-366
dc.identifierhttp://dx.doi.org/10.2166/wh.2017.252
dc.identifier.citationJournal Of Water And Health. London, v. 15, n. 3, p. 360-366, 2017.
dc.identifier.doi10.2166/wh.2017.252
dc.identifier.issn1477-8920
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54360
dc.identifier.wosWOS:000403110200004
dc.language.isoeng
dc.publisherIwa Publishing
dc.relation.ispartofJournal Of Water And Health
dc.rightsAcesso restrito
dc.subjectapoptosisen
dc.subjectcell proliferationen
dc.subjectcell viabilityen
dc.subjectcytotoxicity analysisen
dc.subjectdesalinated seawateren
dc.titleEffects of drinking desalinated seawater on cell viability and proliferationen
dc.typeArtigo
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