Antihypertensive effect of a standardized aqueous extract of Cecropia glaziovii Sneth in rats: An in vivo approach to the hypotensive mechanism

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dc.contributor.authorLima-Landman, M. T. R.
dc.contributor.authorBorges, A. C. R.
dc.contributor.authorCysneiros, Roberta Monterazzo [UNIFESP]
dc.contributor.authorDe Lima, T. C. M.
dc.contributor.authorSouccar, C.
dc.contributor.authorLapa, A. J.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Federal de Santa Catarina (UFSC)
dc.date.accessioned2016-01-24T13:48:39Z
dc.date.available2016-01-24T13:48:39Z
dc.date.issued2007-05-01
dc.description.abstractCecropia glaziovii Sneth is a common tree at the Southeastern Brazilian coast. As many other species of the genus, it shares the reputed folk use to treat heart failure, cough, asthma and bronchitis. the plant has been cultivated under controlled conditions and the 2% aqueous extract (AE) prepared with the dried leaves was standardized by its chemical contents on catechins, flavonoids and procyanidins. the present paper reports the antihypertensive activity of AE and of n-butanol fraction (BuF), an enriched semi-purified butanolic fraction used to isolate the main chemical constituents. Oral administration of AE and BuF induced hypotension in normotensive rats. the effect of AE (0.5 g/ kg/bi, p.o.) was time and dose-dependent peaking at 2-3 weeks after daily administration. BuF was faster but not more active than AE. Both extracts decreased the hypertension of spontaneous hypertensive rats, the hypertension induced in rats by L-NAME treatment and that induced by constriction of one renal artery. the antihypertensive effect was maintained for as long as 60 days of treatment and was reversible upon drug washout at the same rate of its establishment. Acute i.v. administration of BuF to anesthetized rats induced a fast short-lasting hypotension and inhibited the pressor responses to noradrenaline, angiotensin I and angiotensin II by 40%. These results were indirect indications that the hypotension induced by AE is not related to ACE inhibition, increased NO synthesis, or specific blockade of al and ATI receptors. It can be suggested that BuF interferes with the calcium handling mechanisms in smooth muscle cells and neurons. Intravenous injection of five out of nine compounds isolated from BuF produced immediate but short-lasting hypotension that does not correlate with the onset of the hypotension after oral treatment. This finding suggests that they may not be the compounds directly responsible for the delayed and sustained hypotension after per os administration of AE. the many compounds isolated from AE are under evaluation to determine its pharmacokinetics, mechanisms of action and interactions necessary to yield the plant effect. Although its mechanism is still unknown, AE seems to be an effective and safe anti hypertensive phytomedicine. (C) 2007 Elsevier GmbH. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Nat Prod Sect, Dept Pharmacol, Escola Paulista Med, BR-04044020 São Paulo, Brazil
dc.description.affiliationUniv Fed Santa Catarina, Dept Pharmacol, Neuropharmacol Lab, Florianopolis, SC, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Nat Prod Sect, Dept Pharmacol, Escola Paulista Med, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent314-320
dc.identifierhttp://dx.doi.org/10.1016/j.phymed.2007.03.003
dc.identifier.citationPhytomedicine. Jena: Elsevier Gmbh, Urban & Fischer Verlag, v. 14, n. 5, p. 314-320, 2007.
dc.identifier.doi10.1016/j.phymed.2007.03.003
dc.identifier.issn0944-7113
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/29713
dc.identifier.wosWOS:000247200100003
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofPhytomedicine
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectCecropia glazioviien
dc.subjectmedicinal plantsen
dc.subjectantihypertensive activityen
dc.subjectphytomedicineen
dc.titleAntihypertensive effect of a standardized aqueous extract of Cecropia glaziovii Sneth in rats: An in vivo approach to the hypotensive mechanismen
dc.typeinfo:eu-repo/semantics/article
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