Modification of the linker amino acid in the cell-penetrating peptide NickFect55 leads to enhanced pDNA transfection for in vivo applications

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dc.citation.volume15pt_BR
dc.contributor.authorPorosk, Ly
dc.contributor.authorMello, Lucas
dc.contributor.authorda Silva, Emerson Rodrigo [UNIFESP]
dc.contributor.authorHärk, Heleri
dc.contributor.authorKurrikoff, Kaido
dc.contributor.authorArukuust, Piret
dc.contributor.authorLatteshttp://lattes.cnpq.br/7800589206457326pt_BR
dc.date.accessioned2023-05-11T13:36:10Z
dc.date.available2023-05-11T13:36:10Z
dc.date.issued2023-02-20
dc.description.abstractDespite numerous efforts over the last three decades, nucleic acid-based therapeutics still lack delivery platforms in the clinical stage. Cell-penetrating peptides (CPPs) may offer solutions as potential delivery vectors. We have previously shown that designing a “kinked” structure in the peptide backbone resulted in a CPP with efficient in vitro transfection properties. Further optimization of the charge distribution in the C-terminal part of the peptide led to potent in vivo activity with the resultant CPP NickFect55 (NF55). Currently, the impact of the linker amino acid was further investigated in the CPP NF55, with the aim to discover potential transfection reagents for in vivo application. Taking into account the expression of the delivered reporter in the lung tissue of mice, and the cell transfection in the human lung adenocarcinoma cell line, the new peptides NF55-Dap and NF55-Dab* have a high potential for delivering nucleic acid-based therapeutics to treat lung associated diseases, such as adenocarcinoma.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipID19/20907-7pt_BR
dc.description.sponsorshipID2022/3056-6pt_BR
dc.format.extent883pt_BR
dc.identifierhttps://www.mdpi.com/1999-4923/15/3/883
dc.identifier.doi10.3390/pharmaceutics15030883
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/67486
dc.languageporpt_BR
dc.publisherMDPI
dc.relation.ispartofPharmaceutics
dc.rightsinfo:eu-repo/semantics/openAccesspt_BR
dc.subjectCell penetrating peptideen
dc.subjectChemical synthesisen
dc.subjectAtomic force microscopyen
dc.subjectpDNAen
dc.subjectTransfectionen
dc.titleModification of the linker amino acid in the cell-penetrating peptide NickFect55 leads to enhanced pDNA transfection for in vivo applicationsen
dc.typeinfo:eu-repo/semantics/articlept_BR
unifesp.campusEscola Paulista de Medicina (EPM)pt_BR
unifesp.departamentoBiofísicapt_BR
unifesp.graduateProgramCiências Biológicas (Biologia Molecular)pt_BR
unifesp.researchAreaBiofísica molecularpt_BR
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