Dendritic Cells Primed with Paracoccidioides brasiliensis Peptide P10 Are Therapeutic in Immunosuppressed Mice with Paracoccidioidomycosis

Página do item simplificado
dc.citation.volume8]
dc.contributor.authorSilva, Leandro B. R.
dc.contributor.authorDias, Lucas S.
dc.contributor.authorRittner, Glauce M. G.
dc.contributor.authorMunoz, Julian E.
dc.contributor.authorSouza, Ana C. O.
dc.contributor.authorNosanchuk, Joshua D.
dc.contributor.authorTravassos, Luiz R. [UNIFESP]
dc.contributor.authorTaborda, Carlos P.
dc.coverageLausanne
dc.date.accessioned2020-06-26T16:30:36Z
dc.date.available2020-06-26T16:30:36Z
dc.date.issued2017
dc.description.abstractParacoccidioidomycosis (PCM) is an endemic systemic mycosis in Latin America, with the highest prevalence in Brazil, Colombia, and Venezuela. Fungi of the Paracoccidioides genus are etiologic agents of the disease. The 15 amino acid peptide P10 is derived from gp43, the main diagnostic antigen of Paracoccidioides brasiliensis. We previously reported that P10-pulsed dendritic cells (DCs) induce a protective response against P. brasiliensis. Presently, dexamethasone-treated BALB/c mice were intratracheally infected with P. brasiliensis Pb18 to establish the therapeutic efficacy of P10-pulsed DCs. Immunosuppressed and infected animals that received DCs had a reduction in their fungal burden, and this result was most pronounced in mice receiving DCs primed with P10. The efficacy of therapeutic DCs was significantly augmented by concomitant treatment with trimethoprim-sulfamethoxazole. Additionally, primedDCs with or without the antifungal drug induced a beneficial Th1-biased immune response and significantly reduced tissue damage. In conclusion, these studies with immunocompromised mice demonstrate that P10-pulsed DCs with or without concomitant antifungal drugs are potently effective in combating invasive PCM. These findings support further translational studies to validate the use of P10-primed DCs for PCM in immunocompetent and immunosuppressed hosts.en
dc.description.affiliationUniv Sao Paulo, Trop Med Inst USP LIM53, Lab Med Mycol, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo, Brazil
dc.description.affiliationAlbert Einstein Coll Med, Dept Med, Bronx, NY 10467 USA
dc.description.affiliationAlbert Einstein Coll Med, Dept Microbiol & Immunol, Bronx, NY 10467 USA
dc.description.affiliationUniv Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFAPESP
dc.description.sponsorshipCNPq
dc.description.sponsorshipCAPES
dc.description.sponsorshipNIH
dc.description.sponsorshipIDFAPESP: 2016/08730-6
dc.description.sponsorshipIDCNPq
dc.description.sponsorshipIDCAPES
dc.description.sponsorshipIDNIH: AI52733
dc.description.sponsorshipIDNIH: AI1033142-21S
dc.description.sponsorshipIDNIH: AI124797
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.3389/fmicb.2017.01057]
dc.identifier.citationFrontiers In Microbiology. Lausanne, v. 8, p. -, 2017.
dc.identifier.doi10.3389/fmicb.2017.01057
dc.identifier.fileWOS000403219300001.pdf
dc.identifier.issn1664-302X
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/53657
dc.identifier.wosWOS:000403219300001
dc.language.isoeng
dc.publisherFrontiers Media Sa
dc.relation.ispartofFrontiers In Microbiology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectParacoccidioides brasiliensisen
dc.subjectparacoccidioidomycosisen
dc.subjectP10en
dc.subjectadjuvantsen
dc.subjectdendritic cellsen
dc.subjectvaccineen
dc.titleDendritic Cells Primed with Paracoccidioides brasiliensis Peptide P10 Are Therapeutic in Immunosuppressed Mice with Paracoccidioidomycosisen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
WOS000403219300001.pdf
Tamanho:
3.79 MB
Formato:
Adobe Portable Document Format
Descrição:
Baixar
Coleções
EPM - Artigos