YWHAE silencing induces cell proliferation, invasion and migration through the up-regulation of CDC25B and MYC in gastric cancer cells: new insights about YWHAE role in the tumor development and metastasis process

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dc.citation.issue51
dc.citation.volume7
dc.contributor.authorLeal, Mariana Ferreira [UNIFESP]
dc.contributor.authorRibeiro, Helem Ferreira
dc.contributor.authorAntonio Rey, Juan
dc.contributor.authorPinto, Giovanny Reboucas
dc.contributor.authorSmith, Marilia Cardoso [UNIFESP]
dc.contributor.authorMoreira-Nunes, Caroline Aquino
dc.contributor.authorAssumpcao, Paulo Pimentel
dc.contributor.authorLamarao, Leticia Martins
dc.contributor.authorCalcagno, Danielle Queiroz
dc.contributor.authorMontenegro, Raquel Carvalho
dc.contributor.authorBurbano, Rommel Rodriguez
dc.coverageOrchard Park
dc.date.accessioned2020-07-31T12:47:02Z
dc.date.available2020-07-31T12:47:02Z
dc.date.issued2016
dc.description.abstractWe previously observed reduced YWHAE (14-3-3e) protein expression in a small set of gastric cancer samples. YWHAE may act as a negative regulator of the cyclin CDC25B, which is a transcriptional target of MYC oncogene. The understanding of YWHAE role and its targets is important for the better knowledge of gastric carcinogenesis. Thus, we aimed to evaluate the relationship among YWHAE, CDC25B, and MYC in vitro and in vivo. For this, we analyzed the YWHAE, CDC25B, and MYC expression in YWHA-silenced, CDC25B-silenced, and MYC-silenced gastric cancer cell lines, as well as in gastric cancer and non-neoplastic gastric samples. In gastric cancer cell lines, YWHAE was able to inhibit the cell proliferation, invasion and migration through the reduction of MYC and CDC25B expression. Conversely, MYC induced the cell proliferation, invasion and migration through the induction of CDC25B and the reduction of YWHAE. Most of the tumors presented reduced YWHAE and increased CDC25B expression, which seems to be important for tumor development. Increased MYC expression was a common finding in gastric cancer and has a role in poor prognosis. In the tumor initiation, the opposite role of YWHAE and CDC25B in gastric carcinogenesis seems to be independent of MYC expression. However, the inversely correlation between YWHAE and MYC expression seems to be important for gastric cancer cells invasion and migration. The interaction between YWHAE and MYC and the activation of the pathways related to this interaction play a role in the metastasis process.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Morfol Genet, Disciplina Genet, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Ortopedia Traumatol, Sao Paulo, Brazil
dc.description.affiliationHosp Univ Joao Barros Barreto, Nucleo Pesquisas Oncol, Belem, Para, Brazil
dc.description.affiliationFed Univ Para, Inst Ciencias Biol, BR-66059 Belem, Para, Brazil
dc.description.affiliationHosp Univ Paz, Lab Oncogenet Mol, Madrid, Spain
dc.description.affiliationUniv Fed Piaui, Dept Biomed, Parnaiba, Brazil
dc.description.affiliationUniv Fed Ceara, Fac Med, Dept Fisiol & Farmacol, Fortaleza, Ceara, Brazil
dc.description.affiliationFundacao Ctr Hemoterapia & Hematol Para, Lab Testes Acidos Nucl, Belem, Para, Brazil
dc.description.affiliationHosp Ophir Loyola, Mol Biol Lab, Belem, Para, Brazil
dc.description.affiliationUnifespDisciplina de Genética, Departamento de Morfologia e Genética, Universidade Federal de São Paulo, São Paulo, Brazil
dc.description.affiliationUnifespDepartamento de Ortopedia e Traumatologia, Universidade Federal de São Paulo, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent85393-85410
dc.identifierhttp://dx.doi.org/10.18632/oncotarget.13381
dc.identifier.citationOncotarget. Orchard Park, v. 7, n. 51, p. 85393-85410, 2016.
dc.identifier.doi10.18632/oncotarget.13381
dc.identifier.fileWOS000391353200128.pdf
dc.identifier.issn1949-2553
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56542
dc.identifier.wosWOS:000391353200128
dc.language.isoeng
dc.publisherImpact Journals Llc
dc.relation.ispartofOncotarget
dc.rightsAcesso aberto
dc.subjectgastric canceren
dc.subjectcancer developmenten
dc.subjectYWHAEen
dc.subjectCDC25Ben
dc.subjectMYCen
dc.titleYWHAE silencing induces cell proliferation, invasion and migration through the up-regulation of CDC25B and MYC in gastric cancer cells: new insights about YWHAE role in the tumor development and metastasis processen
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