In Vivo Treatments with Fulvestrant and Anastrozole Differentially Affect Gene Expression in the Rat Efferent Ductules

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dc.contributor.authorGomes, Gisele Renata de Oliveira [UNIFESP]
dc.contributor.authorYasuhara, Fabiana [UNIFESP]
dc.contributor.authorSiu, Erica Rosanna [UNIFESP]
dc.contributor.authorFernandes, Sheilla Alessandra Ferreira [UNIFESP]
dc.contributor.authorAvellar, Maria Christina Werneck [UNIFESP]
dc.contributor.authorLazari, Maria de Fatima Magalhaes [UNIFESP]
dc.contributor.authorPorto, Catarina Segreti [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionInst Nacl Farmacol & Biol Mol
dc.date.accessioned2016-01-24T14:05:51Z
dc.date.available2016-01-24T14:05:51Z
dc.date.issued2011-01-01
dc.description.abstractEstrogen plays a key role in maintaining the morphology and function of the efferent ductules We previously demonstrated that the antiestrogen fulvestrant markedly affected gene expression in the rat efferent ductules the mechanism of fulvestrant action to modulate gene expression may involve not only the blockade of ESR1 and ESR2 estrogen receptors, but also the activation of ESR1 and ESR2 when the receptors are tethered to AP 1 or SP1 transcription factors, or the activation of the G protein-coupled estrogen receptor 1 We therefore compared the effects of two strategies to interfere with estrogen action in the rat efferent ductules treatment with fulvestrant or with the aromatase inhibitor anastrozole Whereas fulvestrant markedly increased Mmp7 and Spp1, and reduced Npfx1 mRNA levels, no changes were observed with anastrozole Fulvestrant caused changes in epithelial morphology that were not seen with anastrozole Fulvestrant shifted MMP7 immunolocalization in the epithelial cells from the supranuclear to the apical region, this effect was less pronounced with anastrozole in vitro studies of S-35-methiomne incorporation showed that protein release was increased, whereas tissue protein content in the efferent ductules of fulvestrant-treated rats was decreased Although fulvestrant markedly affected gene expression, no changes were observed on AP 1 and SP1 DNA-binding activity the blockade of ESRs seems to be the major reason explaining the differences between both treatments At least some of the effects of fulvestrant appear to result from compensatory mechanisms activated by the dramatic changes caused by ESR1 blockadeen
dc.description.affiliationUniversidade Federal de São Paulo, Sect Expt Endocrinol, Dept Pharmacol, INFAR, BR-04044020 São Paulo, Brazil
dc.description.affiliationInst Nacl Farmacol & Biol Mol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Sect Expt Endocrinol, Dept Pharmacol, INFAR, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt
dc.description.sponsorshipIDFAPESP: 2008/56564 1pt
dc.format.extent52-61
dc.identifierhttps://dx.doi.org/10.1095/biolreprod.110.085340
dc.identifier.citationBiology of Reproduction. Madison: Soc Study Reproduction, v. 84, n. 1, p. 52-61, 2011.
dc.identifier.doi10.1095/biolreprod.110.085340
dc.identifier.issn0006-3363
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/33193
dc.identifier.wosWOS:000285658600009
dc.language.isoeng
dc.publisherSoc Study Reproduction
dc.relation.ispartofBiology of Reproduction
dc.rightsAcesso aberto
dc.subjectEstradiol receptoren
dc.subjectGene regulationen
dc.subjectMale reproductive tracten
dc.titleIn Vivo Treatments with Fulvestrant and Anastrozole Differentially Affect Gene Expression in the Rat Efferent Ductulesen
dc.typeArtigo
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