Bovine Papillomavirus Clastogenic Effect Analyzed in Comet Assay

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dc.contributor.authorAraldi, Rodrigo Pinheiro
dc.contributor.authorMelo, Thatiana Correa de
dc.contributor.authorDiniz, Nara [UNIFESP]
dc.contributor.authorMazzuchelli-de-Souza, Jacqueline [UNIFESP]
dc.contributor.authorCarvalho, Rodrigo Franco de
dc.contributor.authorBeçak, Willy
dc.contributor.authorStocco, Rita de Cassia
dc.contributor.institutionInst Butantan
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Fed Integracao Latinoamer UNILA
dc.date.accessioned2016-01-24T14:30:54Z
dc.date.available2016-01-24T14:30:54Z
dc.date.issued2013-01-01
dc.description.abstractBovine papillomavirus (BPV) is an oncogenic virus related to serious livestock diseases. Oncoproteins encoded by BPV are involved in several steps of cellular transformation and have been reported as presenting clastogenic effects in peripheral lymphocytes and primary culture cells. the aim of this study was to evaluate the clastogenic potential of BPV types 1, 2, and 4 by comet assay. Peripheral blood was collected from 37 bovines, 32 infected with different levels of papillomatosis (12 animals have no affection) and five calves, virus free (negative control). the viral identification showed presence of more than one virus type in 59.375% of the infected animals. Comet assay was performed according to alkaline technique. the Kruskal-Wallis test showed statistical difference between the negative control group and infected animals (P = 0.0015). the Dunn post hoc test showed difference comparing the infected animals with calves. Mann-Whitney U test verified no difference between animals infected with only one viral type and animals presenting more than one viral type. the comet assay is considered an efficient tool for assessment of damage in the host chromatin due to viral action, specifically highlighting viral activity in blood cells.en
dc.description.affiliationInst Butantan, Genet Lab, BR-05503900 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Ciencias Biomed, Programa Posgrad Interunidades Biotecnol, BR-05508900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Programa Posgrad Biol Estrutural & Func, BR-04023900 São Paulo, Brazil
dc.description.affiliationUniv Fed Integracao Latinoamer UNILA, Dept Biol, BR-85867970 Foz Do Iguacu, PR, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Programa Posgrad Biol Estrutural & Func, BR-04023900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundacao do Desenvolvimento Administrativo (PAP-FUNDAP)
dc.description.sponsorshipIDCNPq: 402539/2011-7
dc.format.extent7
dc.identifierhttps://dx.doi.org/10.1155/2013/630683
dc.identifier.citationBiomed Research International. New York: Hindawi Publishing Corporation, 7 p., 2013.
dc.identifier.doi10.1155/2013/630683
dc.identifier.fileWOS000322495600001.pdf
dc.identifier.issn2314-6133
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/35702
dc.identifier.wosWOS:000322495600001
dc.language.isoeng
dc.publisherHindawi Publishing Corporation
dc.relation.ispartofBiomed Research International
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleBovine Papillomavirus Clastogenic Effect Analyzed in Comet Assayen
dc.typeinfo:eu-repo/semantics/article
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