Expression and in Silico Analysis of the Recombinant Bovine Papillomavirus E6 Protein as a Model for Viral Oncoproteins Studies

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dc.contributor.authorMazzuchelli-de-Souza, Jacqueline
dc.contributor.authorCarvalho, Rodrigo Franco de
dc.contributor.authorRuiz, Renato de Mello
dc.contributor.authorMelo, Thatiana Correa de [UNIFESP]
dc.contributor.authorAraldi, Rodrigo Pinheiro
dc.contributor.authorCarvalho, Eneas de
dc.contributor.authorThompson, Claudia Elizabeth
dc.contributor.authorSircili, Marcelo Palma
dc.contributor.authorBeçak, Willy
dc.contributor.authorStocco, Rita de Cassia
dc.contributor.institutionInst Butantan
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionLab Nacl Computacao Cient
dc.contributor.institutionUniv Fed Integracao Latino Amer UNILA
dc.date.accessioned2016-01-24T14:30:53Z
dc.date.available2016-01-24T14:30:53Z
dc.date.issued2013-01-01
dc.description.abstractBovine papillomaviruses (BPVs) are recognized as the causal agents of economical relevant diseases in cattle, associated with the development of tumors in skin and mucosa. the oncogenesis process is mainly associated with different viral oncoprotein expressions, which are involved in cell transformation. the expression and characterization of recombinant viral oncoproteins represent an attractive strategy to obtain biotechnological products as antibodies and potential vaccines, Thus, the aim of this work was to clone and express the BPV-1 and BPV-2 E6 recombinant proteins and perform in silico analysis in order to develop a strategy for the systematic study of other papillomaviruses oncoproteins. the results demonstrated that BPV-1 and BPV-2 E6 recombinant proteins were expressed and purified from bacterial system as well as its in silico analysis was performed in order to explore and predict biological characteristics of these proteins.en
dc.description.affiliationInst Butantan, Lab Genet, BR-05503900 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Ciencias Biomed, Programa Posgrad Interunidades Biotechnol, BR-05508900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Programa Posgrad Biol Estrutural & Func, BR-04023900 São Paulo, Brazil
dc.description.affiliationInst Butantan, Lab Biotecnol Mol, BR-05503900 São Paulo, Brazil
dc.description.affiliationLab Nacl Computacao Cient, BR-25651075 Petropolis, RJ, Brazil
dc.description.affiliationUniv Fed Integracao Latino Amer UNILA, Dept Biol, BR-85867970 Foz Do Iguacu, PR, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Programa Posgrad Biol Estrutural & Func, BR-04023900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIDCNPq: 554816/2006-7
dc.description.sponsorshipIDCNPq: 402539/2011-7
dc.description.sponsorshipIDFAPESP: 2006/02439-6
dc.format.extent9
dc.identifierhttps://dx.doi.org/10.1155/2013/421398
dc.identifier.citationBiomed Research International. New York: Hindawi Publishing Corporation, 9 p., 2013.
dc.identifier.doi10.1155/2013/421398
dc.identifier.fileWOS000321639300001.pdf
dc.identifier.issn2314-6133
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/35692
dc.identifier.wosWOS:000321639300001
dc.language.isoeng
dc.publisherHindawi Publishing Corporation
dc.relation.ispartofBiomed Research International
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleExpression and in Silico Analysis of the Recombinant Bovine Papillomavirus E6 Protein as a Model for Viral Oncoproteins Studiesen
dc.typeinfo:eu-repo/semantics/article
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