Autologous transplantation of adult adipose derived stem cells into rabbit urethral wall

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dc.contributor.authorAlmeida, Fernando Goncalves [UNIFESP]
dc.contributor.authorDantas Nobre, Yuri Tulio [UNIFESP]
dc.contributor.authorLeite, Katia R.
dc.contributor.authorBruschini, Homero
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T13:59:44Z
dc.date.available2016-01-24T13:59:44Z
dc.date.issued2010-06-01
dc.description.abstractA study was carried out to evaluate the feasibility of autologous adipose derived stem cells (ADSC) transplantation into female rabbits' urethra walls as an alternative to intrinsic urethral regeneration.Inguinal fat pad of 12 New Zealand adult female rabbits were harvested and processed to obtain stromal vascular fraction (SVF). the SVF were platted to isolate ADSC. Before urethral injection, cells were labeled with DiI marker. the urethra wall was injected with 1 x 10(7) autologous cells or saline (sham). the urethra was harvested at 2, 4, and 8 weeks to identify DiI-labeled cells.At 2 and 4 weeks, the ADSCs create a nodule localized in the urethral sub-mucosa. At 8 weeks, the ADSCs spread and integrated with the urethra wall from the initial injection site.This is the first study to demonstrate a successful autologous ADSCs transplantation. It confirms that ADSCs can survive and integrate within the urethral wall.en
dc.description.affiliationUniversidade Federal de São Paulo, Paulista Sch Med, Dept Urol, São Paulo, Brazil
dc.description.affiliationState Univ São Paulo, Sch Med, Dept Urol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Paulista Sch Med, Dept Urol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 06/57479-2
dc.format.extent743-748
dc.identifierhttp://dx.doi.org/10.1007/s00192-009-1090-8
dc.identifier.citationInternational Urogynecology Journal. London: Springer London Ltd, v. 21, n. 6, p. 743-748, 2010.
dc.identifier.doi10.1007/s00192-009-1090-8
dc.identifier.issn0937-3462
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/32575
dc.identifier.wosWOS:000276964000019
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofInternational Urogynecology Journal
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.subjectAdult stem cellen
dc.subjectAnimal modelen
dc.subjectBladderen
dc.subjectTissue engineeringen
dc.subjectUrethra wallen
dc.subjectUrinary incontinenceen
dc.titleAutologous transplantation of adult adipose derived stem cells into rabbit urethral wallen
dc.typeinfo:eu-repo/semantics/article
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