Conditional Deletion of Ric-8b in Olfactory Sensory Neurons Leads to Olfactory Impairment

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dc.citation.issue50
dc.citation.volume37
dc.contributor.authorMachado, Cleiton F.
dc.contributor.authorNagai, Maria H.
dc.contributor.authorLyra, Cassandra S.
dc.contributor.authorReis-Silva, Thiago M.
dc.contributor.authorMachado, Andre M. [UNIFESP]
dc.contributor.authorGlezer, Isaias [UNIFESP]
dc.contributor.authorFelicio, Luciano F.
dc.contributor.authorMalnic, Bettina
dc.coverageWashington
dc.date.accessioned2020-07-02T18:52:19Z
dc.date.available2020-07-02T18:52:19Z
dc.date.issued2017
dc.description.abstractThe olfactory system can discriminate a vast number of odorants. This ability derives from the existence of a large family of odorant receptors expressed in the cilia of the olfactory sensory neurons. Odorant receptors signal through the olfactory-specific G-protein subunit, G alpha olf. Ric-8b, a guanine nucleotide exchange factor, interacts with G alpha olf and can amplify odorant receptor signal transduction in vitro. To explore the function of Ric-8b in vivo, we generated a tissue specific knock-out mouse by crossing OMP-Cre transgenic mice to Ric-8b floxed mice. We found that olfactory-specific Ric-8b knock-out mice of mixed sex do not express the G alpha olf protein in the olfactory epithelium. We also found that in these mice, the mature olfactory sensory neuron layer is reduced, and that olfactory sensory neurons show increased rate of cell death compared with wild-type mice. Finally, behavioral tests showed that the olfactory-specific Ric-8b knock-out mice show an impaired sense of smell, even though their motivation and mobility behaviors remain normal.en
dc.description.affiliationUniv Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Sch Vet Med, Dept Pathol, BR-05508270 Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Escola Paulista Med, Dept Biochem, BR-04023900 Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Escola Paulista Med, Dept Biochem, BR-04023900 Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo [FAPESP] [2016/24471-0, 2012/24640-6, 2014/15495-8]
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico
dc.description.sponsorshipIDFAPESP [2016/24471-0, 2012/24640-6, 2014/15495-8]
dc.format.extent12202-12213
dc.identifierhttp://dx.doi.org/10.1523/JNEUROSCI.0943-17.2017
dc.identifier.citationJournal Of Neuroscience. Washington, v. 37, n. 50, p. 12202-12213, 2017.
dc.identifier.doi10.1523/JNEUROSCI.0943-17.2017
dc.identifier.fileWOS000418054700011.pdf
dc.identifier.issn0270-6474
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/53997
dc.identifier.wosWOS:000418054700011
dc.language.isoeng
dc.publisherSoc Neuroscience
dc.relation.ispartofJournal Of Neuroscience
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectG-proteinen
dc.subjectGEFen
dc.subjectknock-out mouseen
dc.subjectodorant receptoren
dc.subjectolfactionen
dc.subjectolfactory sensory neuronen
dc.titleConditional Deletion of Ric-8b in Olfactory Sensory Neurons Leads to Olfactory Impairmenten
dc.typeinfo:eu-repo/semantics/article
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