Membrane permeabilization induced by Triton X-100: The role of membrane phase state and edge tension

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dc.citation.volume202
dc.contributor.authorMattei, Bruno [UNIFESP]
dc.contributor.authorLira, Rafael Bezerra de [UNIFESP]
dc.contributor.authorPerez, Katia Regina [UNIFESP]
dc.contributor.authorRiske, Karin do Amaral [UNIFESP]
dc.coverageClare
dc.date.accessioned2020-07-31T12:46:56Z
dc.date.available2020-07-31T12:46:56Z
dc.date.issued2017
dc.description.abstractDetergents are widely used to solubilize and separate biomembrane components. It is therefore relevant to study and understand the mechanistic details underlying detergent-lipid interactions using biomimetic systems. Here, we have investigated in detail the process of membrane permeabilization and the nature of pores induced by sub-solubilizing concentrations of the detergent Triton X-100 (TX-100) in bilayers composed of palmitoyl oleoyl phosphatidylcholine (POPC), sphingomyelin (SM) and binary mixtures of these phospholipids with 30 mol% cholesterol (chol). A fluorescence quenching assay was used to evaluate the permeability of large unilamellar vesicles (LUVs) in the presence of increasing concentrations of TX-100. Confocal microscopy was employed to visualize and quantify the permeability of giant unilamellar vesicles (GUVs) to two fluorescent dyes of different sizes in the presence of TX-100. Both methods showed that POPC, POPC/chol and SM membranes become fully permeable at a specific TX-100 concentration, followed by complete (POPC and SM) and partial (POPC/chol) solubilization at a higher detergent concentration. The confocal microscopy experiments revealed that opening of pores occurs as a well-defined event and that for POPC and POPC/chol the pores were initially selective to the small probe and then grew and allowed passage of the larger dye as well. On the other hand, the insoluble SM/chol membranes exhibited only a mild TX-100-induced permeabilization. The membrane edge tension of the liquid phases was measured from the closure rate of macropores induced by electric pulses in GUVs. Membrane edge tension was shown to be sensitive to membrane composition and to decrease in the presence of TX-100. We propose that extensive permeabilization occurs below a critical membrane edge tension, which is eventually reached in the partially and fully soluble compositions, but not in the insoluble mixture. (C) 2016 Elsevier Ireland Ltd. All rights reserved.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Biofis, Sao Paulo, Brazil
dc.description.affiliationUnifespDepartamento de Biofísica, Universidade Federal de São Paulo, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)]
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipINCT-FCx
dc.description.sponsorshipIDFAPESP: 11/22171-6
dc.description.sponsorshipIDFAPESP: 13/20499-0
dc.description.sponsorshipIDCNPq: 472054/2011-2
dc.format.extent28-37
dc.identifierhttp://dx.doi.org/10.1016/j.chemphyslip.2016.11.009
dc.identifier.citationChemistry And Physics Of Lipids. Clare, v. 202, p. 28-37, 2017.
dc.identifier.doi10.1016/j.chemphyslip.2016.11.009
dc.identifier.issn0009-3084
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56471
dc.identifier.wosWOS:000392895100005
dc.language.isoeng
dc.publisherElsevier Ireland Ltd
dc.relation.ispartofChemistry And Physics Of Lipids
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectMembrane permeabilizationen
dc.subjectSolubilizationen
dc.subjectCholesterolen
dc.subjectTriton X-100en
dc.subjectEdge tensionen
dc.subjectGiant unilamellar vesiclesen
dc.titleMembrane permeabilization induced by Triton X-100: The role of membrane phase state and edge tensionen
dc.typeinfo:eu-repo/semantics/article
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