Effect of ionizing radiation exposure on Trypanosoma cruzi ubiquitin-proteasome system

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dc.citation.volume212
dc.contributor.authorCerqueira, Paula Gonçalves
dc.contributor.authorPassos-Silva, Danielle Gomes
dc.contributor.authorVieira-da-Rocha, João Pedro
dc.contributor.authorMendes, Isabela Cecilia
dc.contributor.authorOliveira, Karla Andrade de
dc.contributor.authorOliveira, Camila Franco Batista de
dc.contributor.authorVilela, Liza Figueiredo Felicori
dc.contributor.authorNagem, Ronaldo Alves Pinto
dc.contributor.authorCardoso, Joseane
dc.contributor.authorNardelli, Sheila Cristina
dc.contributor.authorKrieger, Marco Aurélio
dc.contributor.authorFranco, Gloria Regina
dc.contributor.authorMacedo, Andrea Mara
dc.contributor.authorPena, Sergio Danilo Junho
dc.contributor.authorSchenkman, Sergio [UNIFESP]
dc.contributor.authorGomes, Dawidson A.
dc.contributor.authorGuerra-Sa, Renata
dc.contributor.authorMachado, Carlos Renato
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.coverageAmsterdam
dc.date.accessioned2020-07-17T14:02:45Z
dc.date.available2020-07-17T14:02:45Z
dc.date.issued2017
dc.description.abstractIn recent years, proteasome involvement in the damage response induced by ionizing radiation (IR) became evident. However, whether proteasome plays a direct or indirect role in IR-induced damage response still unclear. Trypanosoma cruzi is a human parasite capable of remarkable high tolerance to IR, suggesting a highly efficient damage response system. Here, we investigate the role of T. cruzi proteasome in the damage response induced by IR. We exposed epimastigotes to high doses of gamma ray and we analyzed the expression and subcellular localization of several components of the ubiquitin-proteasome system. We show that proteasome inhibition increases IR-induced cell growth arrest and proteasome-mediated proteolysis is altered after parasite exposure. We observed nuclear accumulation of 19S and 20S proteasome subunits in response to IR treatments. Intriguingly, the dynamic of 19S particle nuclear accumulation was more similar to the dynamic observed for Rad51 nuclear translocation than the observed for 20S. In the other hand, 20S increase and nuclear translocation could be related with an increase of its regulator PA26 and high levels of proteasome-mediated proteolysis in vitro. The intersection between the opposed peaks of 19S and 20S protein levels was marked by nuclear accumulation of both 20S and 19S together with Ubiquitin, suggesting a role of ubiquitin-proteasome system in the nuclear protein turnover at the time. Our results revealed the importance of proteasome-mediated proteolysis in T. cruzi IR-induced damage response suggesting that proteasome is also involved in T. cruzi IR tolerance. Moreover, our data support the possible direct/signaling role of 19S in DNA damage repair. Based on these results, we speculate that spatial and temporal differences between the 19S particle and 20S proteasome controls proteasome multiple roles in IR damage response. (C) 2017 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv Fed Minas Gerais, Inst Ciencias Biol, Dept Bioquim Imunol, Belo Horizonte, MG, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Microbiol Imunol Parasitol, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Ouro Preto, Inst Ciencias Exatas Biol, Dept Ciencias Biol & Nucleo Pesquisa Ciencias Bio, Ouro Preto, Brazil
dc.description.affiliationInst Carlos Chagas, FIOCRUZ, Curitiba, Parana, Brazil
dc.description.affiliationInst Biol Mol Parana, Curitiba, Parana, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Microbiol Imunol Parasitol, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
dc.description.sponsorshipPRONEX
dc.description.sponsorshipNewton Fund/FAPEMIG
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt
dc.description.sponsorshipIDCNPq: 444334/2014-9
dc.description.sponsorshipIDFAPEMIG: APQ-00827-15
dc.format.extent55-67
dc.identifierhttps://dx.doi.org/10.1016/j.molbiopara.2017.01.005
dc.identifier.citationMolecular And Biochemical Parasitology. Amsterdam, v. 212, p. 55-67, 2017.
dc.identifier.doi10.1016/j.molbiopara.2017.01.005
dc.identifier.issn0166-6851
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54992
dc.identifier.wosWOS:000397374700008
dc.language.isoeng
dc.publisherElsevier Science Bv
dc.relation.ispartofMolecular And Biochemical Parasitology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectTrypanosoma cruzien
dc.subjectProteasomeen
dc.subjectIonizing radiationen
dc.titleEffect of ionizing radiation exposure on Trypanosoma cruzi ubiquitin-proteasome systemen
dc.typeinfo:eu-repo/semantics/article
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