Exercise Training and Caloric Restriction Prevent Reduction in Cardiac Ca2+-Handling Protein Profile in Obese Rats

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dc.contributor.authorPaulino, Ellena Christina
dc.contributor.authorBatista Ferreira, Julio Cesar
dc.contributor.authorBechara, Luiz Roberto
dc.contributor.authorTsutsui, Jeane Mike
dc.contributor.authorMathias Junior, Wilson [UNIFESP]
dc.contributor.authorLima, Fabio Bessa
dc.contributor.authorCasarini, Dulce Elena [UNIFESP]
dc.contributor.authorCicogna, Antonio Carlos
dc.contributor.authorBrum, Patricia Chakur
dc.contributor.authorNegrao, Carlos Eduardo
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:05:29Z
dc.date.available2016-01-24T14:05:29Z
dc.date.issued2010-10-01
dc.description.abstractPrevious studies show that exercise training and caloric restriction improve cardiac function in obesity. However, the molecular mechanisms underlying this effect on cardiac function remain unknown. Thus, we studied the effect of exercise training and/or caloric restriction on cardiac function and Ca2+ handling protein expression in obese rats. To accomplish this goal, male rats fed with a high-fat and sucrose diet for 25 weeks were randomly assigned into 4 groups: high-fat and sucrose diet, high-fat and sucrose diet and exercise training, caloric restriction, and exercise training and caloric restriction. An additional lean group was studied. the study was conducted for 10 weeks. Cardiac function was evaluated by echocardiography and Ca2+ handling protein expression by Western blotting. Our results showed that visceral fat mass, circulating leptin, epinephrine, and norepinephrine levels were higher in rats on the high-fat and sucrose diet compared with the lean rats. Cardiac nitrate levels, reduced/oxidized glutathione, left ventricular fractional shortening, and protein expression of phosphorylated Ser(2808)-ryanodine receptor and Thr(17-)phospholamban were lower in rats on the high-fat and sucrose diet compared with lean rats. Exercise training and/or caloric restriction prevented increases in visceral fat mass, circulating leptin, epinephrine, and norepinephrine levels and prevented reduction in cardiac nitrate levels and reduced: oxidized glutathione ratio. Exercise training and/or caloric restriction prevented reduction in left ventricular fractional shortening and in phosphorylation of the Ser(2808)-ryanodine receptor and Thr(17)-phospholamban. These findings show that exercise training and/or caloric restriction prevent cardiac dysfunction in high-fat and sucrose diet rats, which seems to be attributed to decreased circulating neurohormone levels. in addition, this nonpharmacological paradigm prevents a reduction in the Ser(2808)-ryanodine receptor and Thr(17-)phospholamban phosphorylation and redox status. (Hypertension. 2010;56:629-635.)en
dc.description.affiliationUniv São Paulo, Sch Med, Inst Heart, InCor, BR-05403900 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Sch Med, Inst Biomed Sci, BR-05403900 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Sch Phys Educ & Sport, BR-05403900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Div Nephrol, Dept Med, São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, São Paulo State Univ, Sch Med, Dept Med & Cardiol, Botucatu, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Div Nephrol, Dept Med, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDCNPq: 478463/2006-5
dc.description.sponsorshipIDCNPq: 302146/2007-5
dc.description.sponsorshipIDCNPq: 301519/2008-0
dc.description.sponsorshipIDFAPESP: 06/50851-3
dc.description.sponsorshipIDFAPESP: 2009/03143-1
dc.format.extent629-U137
dc.identifierhttp://dx.doi.org/10.1161/HYPERTENSIONAHA.110.156141
dc.identifier.citationHypertension. Philadelphia: Lippincott Williams & Wilkins, v. 56, n. 4, p. 629-U137, 2010.
dc.identifier.doi10.1161/HYPERTENSIONAHA.110.156141
dc.identifier.issn0194-911X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/32926
dc.identifier.wosWOS:000281881400015
dc.language.isoeng
dc.publisherLippincott Williams & Wilkins
dc.relation.ispartofHypertension
dc.rightsAcesso aberto
dc.subjectlisten
dc.subjectobesityen
dc.subjectexercise trainingen
dc.subjectcaloric restrictionen
dc.subjectcardiac dysfunctionen
dc.subjectCa2+ handlingen
dc.titleExercise Training and Caloric Restriction Prevent Reduction in Cardiac Ca2+-Handling Protein Profile in Obese Ratsen
dc.typeArtigo
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