Genotoxicity assessment of the antimalarial compound artesunate in somatic cells of mice

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dc.contributor.authorAquino, Ivani
dc.contributor.authorPerazzo, Fábio Ferreira [UNIFESP]
dc.contributor.authorMaistro, Edson Luis
dc.contributor.institutionUniv Estadual Paulista
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:16:46Z
dc.date.available2016-01-24T14:16:46Z
dc.date.issued2011-06-01
dc.description.abstractArtesunate is a derivate of artemisinin that is both an antimalarial agent and acts cytotoxically on tumor cells. Despite its therapeutic use, its in vivo genotoxic potential has still not been evaluated. This study, therefore, was an investigation into the effects of a single oral administration of artesunate with an in vivo comet assay that analyzed leukocytes from peripheral blood and liver cells, and a micronucleus (MN) assay of bone marrow cells from male Swiss mice. the artesunate was administered by oral gavage at doses of 5, 50 and 100 mg/kg. Cytotoxicity was assessed by scoring 200 consecutive polychromatic (PCE) and normochromatic (NCE) erythrocytes (PCE/NCE ratio). the results demonstrate that artesunate induced significant DNA damage only in liver cells and that high doses of artesunate caused an increase in the mean number of micronucleated polychromatic erythrocytes (MNPCE). Under our experimental conditions, artesunate showed weak genotoxic effects at low doses and clastogenic effects at high doses. the PCE/NCE ratio indicated no cytotoxicity. the data obtained suggest caution about either continuous or high-dose use of artesunate by humans. (C) 2011 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv Estadual Paulista, UNESP, Fac Filosofia & Ciencias, Dept Fonoaudiol, BR-17525900 Marilia, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, UNESP, Inst Biociencias, Programa Posgrad Biol Geral & Aplicada, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo Unifesp, Dept Ciencias Exatas & Terra, BR-09972270 Diadema, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo Unifesp, Dept Ciencias Exatas & Terra, BR-09972270 Diadema, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDCNPq: 306544/2006-7
dc.description.sponsorshipIDFAPESP: 2008/51175-7
dc.format.extent1335-1339
dc.identifierhttp://dx.doi.org/10.1016/j.fct.2011.03.016
dc.identifier.citationFood and Chemical Toxicology. Oxford: Pergamon-Elsevier B.V., v. 49, n. 6, p. 1335-1339, 2011.
dc.identifier.doi10.1016/j.fct.2011.03.016
dc.identifier.fileWOS000291514800021.pdf
dc.identifier.issn0278-6915
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/33716
dc.identifier.wosWOS:000291514800021
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofFood and Chemical Toxicology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectArtesunateen
dc.subjectArtemisinin derivateen
dc.subjectMicronucleus test comet assayen
dc.subjectClastogenicityen
dc.titleGenotoxicity assessment of the antimalarial compound artesunate in somatic cells of miceen
dc.typeinfo:eu-repo/semantics/article
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