The first pacifastin elastase inhibitor characterized from a blood sucking animal

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dc.contributor.authorMarco, Renato de [UNIFESP]
dc.contributor.authorLovato, Diogo V. [UNIFESP]
dc.contributor.authorTorquato, Ricardo J. S. [UNIFESP]
dc.contributor.authorClara, Renan O. [UNIFESP]
dc.contributor.authorBuarque, Diego S. [UNIFESP]
dc.contributor.authorTanaka, Aparecida S. [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:05:08Z
dc.date.available2016-01-24T14:05:08Z
dc.date.issued2010-07-01
dc.description.abstractPacifastin-like protease inhibitors belong to a recent classified protease inhibitor family and they are the smallest protease inhibitors described in animals. in this work, we purified and characterized, for the first time, two neutrophil elastase inhibitors belonging to the pacifastin family from the blood sucking insect Triatoma infestans eggs. the inhibitors showed the same N-terminal sequences, molecular masses of 4257 and 4024 Da by MALDI-TOF mass spectrometry and dissociation constants (Ki) for neutrophil elastase of 0.52 and 0.29 nM, respectively. Using a fat body cDNA library, we cloned a pacifastin precursor containing two protease inhibitor domains similar to locust pacifastins. the first pacifastin domain translated to T. infestans purified protein, named TIPI1. Recombinant TIPI1 expressed in Pichia pastoris system showed similar inhibitory activities compared to the native inhibitor. Its precursor, called TIPP1, is mainly expressed in fat body, and it is up-regulated after blood feeding. the immune challenges of 1(a) instar T. infestans nymph with bacteria or dsRNA strongly stimulated TiPP1 expression in fat body, suggesting a possible role of TiPP1 in T. infestans immunity. This work is the first to characterize a blood feeding insect pacifastin inhibitor. (C) 2010 Elsevier Inc. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipINCT-Entomologia Molecular
dc.description.sponsorshipIDFAPESP: 02/13960-8
dc.description.sponsorshipIDFAPESP: 05/03514-9
dc.description.sponsorshipIDFAPESP: 05/03339-2
dc.description.sponsorshipIDFAPESP: 09/50434-1
dc.description.sponsorshipIDCNPq: 470297/2006-9
dc.format.extent1280-1286
dc.identifierhttp://dx.doi.org/10.1016/j.peptides.2010.03.033
dc.identifier.citationPeptides. New York: Elsevier B.V., v. 31, n. 7, p. 1280-1286, 2010.
dc.identifier.doi10.1016/j.peptides.2010.03.033
dc.identifier.issn0196-9781
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/32683
dc.identifier.wosWOS:000279078700008
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofPeptides
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectPacifastinen
dc.subjectSerine protease inhibitorsen
dc.subjectHematophagousen
dc.subjectImmune responseen
dc.titleThe first pacifastin elastase inhibitor characterized from a blood sucking animalen
dc.typeinfo:eu-repo/semantics/article
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