Administration of Neural Precursor Cells Ameliorates Renal Ischemia-Reperfusion Injury

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dc.contributor.authorWang, Pamella Huey Mei [UNIFESP]
dc.contributor.authorSchwindt, Telma Tiemi [UNIFESP]
dc.contributor.authorBarnabe, Gabriela Filoso [UNIFESP]
dc.contributor.authorMotta, Fabiana Louise Teixeira [UNIFESP]
dc.contributor.authorSemedo, Patricia [UNIFESP]
dc.contributor.authorBeraldo, Felipe Caetano
dc.contributor.authorMazzali, Marilda
dc.contributor.authorReis, Marlene Antonia dos
dc.contributor.authorTeixeira, Vicente de Paula Antunes
dc.contributor.authorPacheco-Silva, Alvaro [UNIFESP]
dc.contributor.authorMello, Luiz Eugenio Araujo de Moraes [UNIFESP]
dc.contributor.authorCamara, Niels Olsen Saraiva [UNIFESP]
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniv Fed Triangulo Mineiro
dc.contributor.institutionInst Israelita Ensino & Pesquisa Albert Einstein
dc.date.accessioned2016-01-24T13:52:00Z
dc.date.available2016-01-24T13:52:00Z
dc.date.issued2009-01-01
dc.description.abstractIn this study we evaluated whether administration of stem cells of neural origin (neural precursor cells, NPCs) could be protective against renal ischemia-reperfusion injury (IRI). We hypothesized that stem cell outcomes are not tissue-specific and that NPCs can improve tissue damage through paracrine mechanisms, especially due to immunomodulation. To this end, Wistar rats (200-250 g) were submitted to 1-hour ischemia and treated with NPCs (4 x 10(6) cells/animal) at 4 h of reperfusion. To serve as controls, ischemic animals were treated with cerebellum homogenate harvested from adult rat brain. All groups were sacrificed at 24 h of reperfusion. NPCs were isolated from rat fetus telencephalon and cultured until neurosphere formation (7 days). Before administration, NPCs were labeled with carboxyfluorescein diacetate succinimydylester (CFSE). Kidneys were harvested for analysis of cytokine profile and macrophage infiltration. At 24 h, NPC treatment resulted in a significant reduction in serum creatinine (IRI + NPC 1.21 + 0.18 vs. IRI 3.33 + 0.14 and IRI + cerebellum 2.95 + 0.78mg/dl, p < 0.05) and acute tubular necrosis (IRI + NPC 46.0 + 2.4% vs. IRI 79.7 + 14.2%, p < 0.05). NPC-CFSE and glial fibrillary acidic protein (GFAP)-positive cells (astrocyte marker) were found exclusively in renal parenchyma, which also presented GFAP and SOX-2 (an embryonic neural stem cell marker) mRNA expression. NPC treatment resulted in lower renal proinflammatory IL1-beta and TNF-alpha expression and higher anti-inflammatory IL-4 and IL-10 transcription. NPC-treated animals also had less macrophage infiltration and decreased serum proinflammatory cytokines (IL-1 beta, TNF-alpha and INF-gamma). Our data suggested that NPC therapy improved renal function by influencing immunological responses. Copyright (C) 2009 S. Karger AG, Baselen
dc.description.affiliationUniv São Paulo, Dept Immunol, Inst Biomed Sci, Lab Transplantat Immunobiol, BR-05508900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Lab Imunol Clin & Expt, Div Nephrol, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Neurophysiol Lab, Div Physiol, São Paulo, Brazil
dc.description.affiliationUniv Estadual Campinas, Div Nephrol, Campinas, SP, Brazil
dc.description.affiliationUniv Fed Triangulo Mineiro, Div Pathol, Belo Horizonte, MG, Brazil
dc.description.affiliationInst Israelita Ensino & Pesquisa Albert Einstein, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Lab Imunol Clin & Expt, Div Nephrol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Neurophysiol Lab, Div Physiol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipMCT/CT-Saude/Decit/SCTIE/MS
dc.description.sponsorshipFundacao de Apoio a Pesquisa do Estado de São Paulo
dc.description.sponsorshipIDMCT/CT-Saude/Decit/SCTIE/MS: 552307/2005-0
dc.description.sponsorshipIDFundacao de Apoio a Pesquisa do Estado de São Paulo: 04/08311-6
dc.description.sponsorshipIDFundacao de Apoio a Pesquisa do Estado de São Paulo: 04/13826-5
dc.description.sponsorshipIDFundacao de Apoio a Pesquisa do Estado de São Paulo: 05/50085-6
dc.description.sponsorshipIDFundacao de Apoio a Pesquisa do Estado de São Paulo: 07/07139-3
dc.format.extentE20-E28
dc.identifierhttp://dx.doi.org/10.1159/000210575
dc.identifier.citationNephron Experimental Nephrology. Basel: Karger, v. 112, n. 1, p. E20-E28, 2009.
dc.identifier.doi10.1159/000210575
dc.identifier.issn1660-2129
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/31117
dc.identifier.wosWOS:000265628600003
dc.language.isoeng
dc.publisherKarger
dc.relation.ispartofNephron Experimental Nephrology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://www.karger.com/Services/RightsPermissions
dc.subjectNeurospheresen
dc.subjectNeural precursor cellsen
dc.subjectIschemia and reperfusion injury, renalen
dc.subjectImmunomodulationen
dc.titleAdministration of Neural Precursor Cells Ameliorates Renal Ischemia-Reperfusion Injuryen
dc.typeinfo:eu-repo/semantics/article
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