Effect of transgastric peritoneal access on peritoneal innate cellular immunity: experimental study in swine

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dc.contributor.authorRodrigues, Rodrigo [UNIFESP]
dc.contributor.authorRezende, Marcelo [UNIFESP]
dc.contributor.authorGomes, Gustavo [UNIFESP]
dc.contributor.authorSouza, Fernando
dc.contributor.authorBlagitz, Maiara
dc.contributor.authorDella Libera, Alice
dc.contributor.authorTaha, Murched [UNIFESP]
dc.contributor.authorFerrari, Angelo Paulo [UNIFESP]
dc.contributor.authorDella Libera, Ermelindo [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionFleury Med & Saude
dc.contributor.institutionHosp Israelita Albert Einstein
dc.date.accessioned2016-01-24T14:31:19Z
dc.date.available2016-01-24T14:31:19Z
dc.date.issued2013-03-01
dc.description.abstractOne of the main concerns of natural orifice surgery is the local and systemic impact on physiology. Few studies have compared natural orifice transluminal endoscopic surgery (NOTES) with other surgical modalities. Most studies are based on systemic variables such as postoperative serum cytokines, with conflicting results. Surgical trauma induces an early inflammatory response, release of cytokines, and local leukocyte activation and oxidative burst. Major surgical trauma is related to impairment of phagocytic function and an increase in production of active oxygen species by phagocytes. the aim of this study was to evaluate the impact of transgastric peritoneoscopy on peritoneal innate immune response compared with laparoscopy and laparotomy in swine.Thirty-four male Sus scrofa domesticus swine were assigned to four groups: transgastric peritoneoscopy (13), laparoscopy (7), laparotomy (7), and sham procedure (7). Twenty-four hours after the procedure, peritoneal fluid cells were harvested by peritoneal washing after necropsy. Flow cytometry analysis of labeled S. aureus and E. coli phagocytosis by peritoneal neutrophils and macrophages was blindly performed. Oxidative burst activity measured by H2O2 production under different challenges was also evaluated.Total operative time varied between all groups. the transgastric, laparoscopy, and laparotomy groups required 56, 17.2, and 40.3 min of mean operative time, respectively (p < 0.05). Even though the mean percentage and intensity of phagocytosis by peritoneal phagocytes were higher in the sham, transgastric, and laparoscopy groups, there was no significant difference between these groups and laparotomy. Macrophage production of H2O2 has been shown to be similar among the transgastric, laparoscopy, and sham groups, and smaller than that in laparotomy (p < 0.05), either under basal conditions, while performing E. coli phagocytosis, or challenged by the presence of E. coli membrane lipopolysaccharide.Under the conditions of this study, transgastric peritoneoscopy has been shown to have minimal impact on peritoneal innate immune response.en
dc.description.affiliationSão Paulo Fed Univ UNIFESP, Dept Med, Clin Gastroenterol Div, São Paulo, Brazil
dc.description.affiliationFleury Med & Saude, Endoscopy Unit, São Paulo, Brazil
dc.description.affiliationSão Paulo Fed Univ UNIFESP, Dept Surg, Expt Surg Div, São Paulo, Brazil
dc.description.affiliationHosp Israelita Albert Einstein, Endoscopy Unit, São Paulo, Brazil
dc.description.affiliationUnifespSão Paulo Fed Univ UNIFESP, Dept Med, Clin Gastroenterol Div, São Paulo, Brazil
dc.description.affiliationUnifespSão Paulo Fed Univ UNIFESP, Dept Surg, Expt Surg Div, São Paulo, Brazil
dc.description.provenanceMade available in DSpace on 2016-01-24T14:31:19Z (GMT). No. of bitstreams: 0 Previous issue date: 2013-03-01en
dc.description.sourceWeb of Science
dc.format.extent964-970
dc.identifierhttp://dx.doi.org/10.1007/s00464-012-2541-8
dc.identifier.citationSurgical Endoscopy and Other Interventional Techniques. New York: Springer, v. 27, n. 3, p. 964-970, 2013.
dc.identifier.doi10.1007/s00464-012-2541-8
dc.identifier.issn0930-2794
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/36023
dc.identifier.wosWOS:000315145500036
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofSurgical Endoscopy and Other Interventional Techniques
dc.rightsAcesso restrito
dc.rights.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.subjectNatural orifice endoscopic surgeryen
dc.subjectLaparoscopyen
dc.subjectImmunityen
dc.subjectInnateen
dc.subjectFlow cytometryen
dc.subjectFree radicalsen
dc.subjectPeritoneumen
dc.titleEffect of transgastric peritoneal access on peritoneal innate cellular immunity: experimental study in swineen
dc.typeArtigo
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