Glycosaminoglycans affect the action of human plasma kallikrein on kininogen hydrolysis and inflammation

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dc.contributor.authorAndrezza, J. G.
dc.contributor.authorNunes, V. A.
dc.contributor.authorCarmona, Adriana Karaoglanovic [UNIFESP]
dc.contributor.authorNader, Helena Bonciani [UNIFESP]
dc.contributor.authorDietrich, Carl Peter [UNIFESP]
dc.contributor.authorSilveira, Vera Lucia Flor [UNIFESP]
dc.contributor.authorShimamoto, K.
dc.contributor.authorUra, N.
dc.contributor.authorSampaio, Misako Uemura [UNIFESP]
dc.contributor.authorSampaio, Claudio Augusto Machado [UNIFESP]
dc.contributor.authorAraujo, M. S.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionSapporo Med Univ
dc.date.accessioned2018-06-15T17:44:16Z
dc.date.available2018-06-15T17:44:16Z
dc.date.issued2002-12-01
dc.description.abstractHuman plasma kallikrein (huPK) is a serine proteinase involved in many biological processes including those of the kallikrein-kinin system. The action of huPK on kininogen results in bradykinin (BK) release, a potent mediator of inflammatory responses. BK generation may be influenced by several agents, and the aim of this work was to investigate the effect of glycosaminoglycans (GAGs) on human high-molecular-weight kininogen (HK) hydrolysis by huPK and on inflammation.huPK was pre-incubated in the absence and presence of different GAGs, followed by the addition of kininogen. Bradykinin released at different times was measured by radioimmunoassay, and K-M and k(cat) were calculated. Tuna and bovine dermatan sulfates, the most potent GAGs studied, reduced by 80% and 68%. respectively, the catalytic efficiency of huPK (control=4.1 x 10(4) M-1 s(-1)) in BK release.The effect of bovine dermatan sulfate (BDS) on inflammatory response was studied in rat paw edema induced by carrageenin and hourly determined (1-4 h) by plethysmography. BDS significantly reduced the inflammatory response in the first and second hours of measurements (24% and 28%, respectively), p < 0.05.GAGs were shown to reduce bradykinin release in vitro and in an inflammation model. This reduction may play a role in the control or maintenance of some pathological and physiological processes. (C) 2002 Published by Elsevier Science B.V.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Biochem, EPM, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Biophys, EPM, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Physiol, EPM, Sao Paulo, Brazil
dc.description.affiliationSapporo Med Univ, Sapporo, Hokkaido, Japan
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Biochem, EPM, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Biophys, EPM, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Physiol, EPM, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent1861-1865
dc.identifierhttps://doi.org/10.1016/S1567-5769(02)00145-5
dc.identifier.citationInternational Immunopharmacology. Amsterdam: Elsevier Science Bv, v. 2, n. 13-14, p. 1861-1865, 2002.
dc.identifier.doi10.1016/S1567-5769(02)00145-5
dc.identifier.issn1567-5769
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/44019
dc.identifier.wosWOS:000179753500017
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofInternational Immunopharmacology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjecthuman plasma kallikreinen
dc.subjecthigh-molecular-weight kininogenen
dc.subjectbradykininen
dc.subjectglycosaminoglycansen
dc.subjectpaw edemaen
dc.titleGlycosaminoglycans affect the action of human plasma kallikrein on kininogen hydrolysis and inflammationen
dc.typeinfo:eu-repo/semantics/article
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