Intraclonal Genome Stability of the Metallo-beta-lactamase SPM-1-producing Pseudomonas aeruginosa ST277, an Endemic Clone Disseminated in Brazilian Hospitals

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dc.citation.volume7
dc.contributor.authorNascimento, Ana P. B.
dc.contributor.authorOrtiz, Mauro F.
dc.contributor.authorMartins, Willames Marcos Brasileiro da Silva [UNIFESP]
dc.contributor.authorMorais, Guilherme L.
dc.contributor.authorFehlberg, Lorena Cristina Corrêa [UNIFESP]
dc.contributor.authorAlmeida, Luiz G. P.
dc.contributor.authorCiapina, Luciane P.
dc.contributor.authorGales, Ana Cristina [UNIFESP]
dc.contributor.authorVasconcelos, Ana T. R.
dc.coverageLausanne
dc.date.accessioned2020-07-31T12:47:04Z
dc.date.available2020-07-31T12:47:04Z
dc.date.issued2016
dc.description.abstractCarbapenems represent the mainstay therapy for the treatment of serious P aeruginosa infections. However, the emergence of carbapenem resistance has jeopardized the clinical use of this important class of compounds. The production of SPM-1 metallo-beta-lactamase has been the most common mechanism of carbapenem resistance identified in P. aeruginosa isolated from Brazilian medical centers. Interestingly, a single SPM-1-producing P. aeruginosa clone belonging to the ST277 has been widely spread within the Brazilian territory. In the current study, we performed a next-generation sequencing of six SPM-1-producing P. aeruginosa ST277 isolates. The core genome contains 5899 coding genes relative to the reference strain P. aeruginosa PAO1. A total of 26 genomic islands were detected in these isolates. We identified remarkable elements inside these genomic islands, such as copies of the bla(spM-1) gene conferring resistance to carbapenems and a type I-C CRISPR-Cas system, which is involved in protection of the chromosome against foreign DNA. In addition, we identified single nucleotide polymorphisms causing amino acid changes in antimicrobial resistance and virulence-related genes. Together,these factors could contribute to the marked resistance and persistence of the SPM-1-producing P aeruginosa ST277 clone. A comparison of the SPM-1-producing P. aeruginosa ST277 genomes showed that their core genome has a high level nucleotide similarity and synteny conservation. The variability observed was mainly due to acquisition of genomic islands carrying several antibiotic resistance genes.en
dc.description.affiliationLab Nacl Comp Cient, Lab Bioinformat, Petropolis, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Escola Paulista Med, Dept Internal Med, Lab Alerta,Div Infect Dis, Sao Paulo, Brazil
dc.description.affiliationUnifespLaboratório Alerta, Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIDCNPq: 305535/2014-5
dc.description.sponsorshipIDCNPq: 302768/2011-4
dc.description.sponsorshipIDCNPq: 312864/2015-9
dc.description.sponsorshipIDFAPERJ: E-26/202.903/2016
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.3389/fmicb.2016.01946
dc.identifier.citationFrontiers In Microbiology. Lausanne, v. 7, p. -, 2016.
dc.identifier.doi10.3389/fmicb.2016.01946
dc.identifier.fileWOS000389268100001.pdf
dc.identifier.issn1664-302X
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56558
dc.identifier.wosWOS:000389268100001
dc.language.isoeng
dc.publisherFrontiers Media Sa
dc.relation.ispartofFrontiers In Microbiology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectdrug resistanceen
dc.subjectcomparative genomicsen
dc.subjectpathogenic bacteriaen
dc.subjectantimicrobial resistanceen
dc.subjectcarbapenemaseen
dc.subjectGram-negative bacillien
dc.titleIntraclonal Genome Stability of the Metallo-beta-lactamase SPM-1-producing Pseudomonas aeruginosa ST277, an Endemic Clone Disseminated in Brazilian Hospitalsen
dc.typeinfo:eu-repo/semantics/article
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