Lack of evidence for regulation of cardiac P-type ATPases and MAP kinases in transgenic mice with cardiac-specific overexpression of constitutively active α1B-adrenoceptors

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dc.contributor.authorBarreto, Frederico
dc.contributor.authorRezende, Daniele Campos
dc.contributor.authorScaramello, Christianne Bretas Vieira
dc.contributor.authorSilva, Claudia Lucia Martins
dc.contributor.authorCunha, Valeria do Monti Nascimento
dc.contributor.authorCaricati-Neto, Afonso [UNIFESP]
dc.contributor.authorJurkiewicz, Aron [UNIFESP]
dc.contributor.authorNoël, Francois
dc.contributor.authorQuintas, Luis Eduardo Menezes
dc.contributor.institutionUniversidade Federal do Rio de Janeiro Instituto de Ciências Biomédicas Programa de Farmacologia
dc.contributor.institutionUniversidade Federal Fluminense Instituto Biomédico Departamento de Fisiologia e Farmacologia
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2015-06-14T13:41:38Z
dc.date.available2015-06-14T13:41:38Z
dc.date.issued2010-05-01
dc.description.abstractThe regulatory function of α1B-adrenoceptors in mammalian heart homeostasis is controversial. The objective of the present study was to characterize the expression/activity of key proteins implicated in cardiac calcium handling (Na+/K+-ATPase and Ca2+-ATPases) and growth (ERK1/2, JNK1/2 and p38) in mice with cardiac-selective overexpression of constitutively active mutant α1B-adrenoceptor (CAMα1B-AR), which present a mild cardiac hypertrophy phenotype. Immunoblot assays showed that myocardial plasma membrane Ca2+-ATPase (PMCA) expression was increased by 30% in CAMα1B-AR mice (N = 6, P < 0.05), although there was no change in sarco/endoplasmic reticulum Ca2+-ATPase (SERCA2) expression. Moreover, total Ca2+-ATPase activity was not modified, but a significant increase in the activity of the thapsigargin-resistant (PMCA) to thapsigargin-sensitive (SERCA) ratio was detected. Neither Na+/K+-ATPase activity nor the expression of α1 and α2 subunit isoforms was changed in CAMα1B-AR mouse hearts. Moreover, immunoblot assays did not provide evidence for an enhanced activation of the three mitogen-activated protein kinases studied in this stage of hypertrophy. Therefore, these findings indicate that chronic cardiac α1B-AR activation in vivo led to mild hypertrophy devoid of significant signs of adaptive modifications concerning primary intracellular calcium control and growth-related proteins, suggesting a minor pathophysiological role of this adrenergic receptor in mouse heart at this stage of development.en
dc.description.affiliationUniversidade Federal do Rio de Janeiro Instituto de Ciências Biomédicas Programa de Farmacologia
dc.description.affiliationUniversidade Federal Fluminense Instituto Biomédico Departamento de Fisiologia e Farmacologia
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Departamento de Farmacologia
dc.description.affiliationUnifespUNIFESP, Depto. de Farmacologia
dc.description.sourceSciELO
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent500-505
dc.identifierhttp://dx.doi.org/10.1590/S0100-879X2010007500028
dc.identifier.citationBrazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 43, n. 5, p. 500-505, 2010.
dc.identifier.doi10.1590/S0100-879X2010007500028
dc.identifier.fileS0100-879X2010000500012.pdf
dc.identifier.issn0100-879X
dc.identifier.scieloS0100-879X2010000500012
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/5700
dc.identifier.wosWOS:000277830900012
dc.language.isoeng
dc.publisherAssociação Brasileira de Divulgação Científica
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.rightsAcesso aberto
dc.subjectα1B-adrenoceptorsen
dc.subjectCa2+-ATPasesen
dc.subjectCardiac hypertrophyen
dc.subjectNa+/K+-ATPaseen
dc.subjectMitogen-activated protein kinasesen
dc.titleLack of evidence for regulation of cardiac P-type ATPases and MAP kinases in transgenic mice with cardiac-specific overexpression of constitutively active α1B-adrenoceptorsen
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