Divergence of HIV-1 quasispecies in an epidemiologic cluster

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dc.contributor.authorDiaz, Ricardo Sobhie [UNIFESP]
dc.contributor.authorZhang, L. Q.
dc.contributor.authorBusch, Michael P. [UNIFESP]
dc.contributor.authorMosley, J. W.
dc.contributor.authorMayer, A.
dc.contributor.institutionIRWIN MEM BLOOD CTR
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionAARON DIAMOND AIDS RES CTR
dc.contributor.institutionUNIV CALIF SAN FRANCISCO
dc.contributor.institutionUNIV SO CALIF
dc.date.accessioned2016-01-24T12:30:19Z
dc.date.available2016-01-24T12:30:19Z
dc.date.issued1997-03-15
dc.description.abstractBackground: During treatment with blood components prepared from an HIV-infected donation, two recipients became infected in 1985. One recipient infected her sexual partner.Objective: To evaluate the evolution of the originally-shared HIV-1 quasispecies in different human hosts over lime, sequence data were obtained from serum from the actual donation sample of blood, and from plasma samples collected from the four members of the epidemiologic cluster over a period extending from 1986 to 1993.Methods: the V3 hypervariable region of env and the gag p17 gene were analysed. CD4 and CD8 counts, as well as HIV RNA burden data, were collected.Results: One patient died from AIDS during the study. This patient showed a greater degree of diversity in the V3 region, with a higher positive charge over time, than the other individuals. Phylogenetic analysis revealed that the V3 sequences from each of the four individuals occupied separate branches of a phylogenetic reconstruction (tree). Two distinct subgroups evolved in the donor, one with GPGR and the other with GSGR/GSGK at the tip of the V3 loop. This latter group was not detected in the other individuals. the sequences in the sexual partner were no more related to those in the infecting transfusion recipient than to sequences from the other members of the cluster, consistent with sexual transmission having occurred at a time shortly after the recipient was infected.Conclusion: the shared HIV-1 quasispecies in this epidemiologic cluster diverged in an individual-specific manner.en
dc.description.affiliationIRWIN MEM BLOOD CTR,RES & SCI SERV,SAN FRANCISCO,CA 94118
dc.description.affiliationUniversidade Federal de São Paulo,ESCOLA PAULISTA MED,São Paulo,BRAZIL
dc.description.affiliationAARON DIAMOND AIDS RES CTR,NEW YORK,NY
dc.description.affiliationUNIV CALIF SAN FRANCISCO,DEPT LAB MED,SAN FRANCISCO,CA 94143
dc.description.affiliationUNIV SO CALIF,SCH MED,LOS ANGELES,CA
dc.description.affiliationUnifespUniversidade Federal de São Paulo,ESCOLA PAULISTA MED,São Paulo,BRAZIL
dc.description.sourceWeb of Science
dc.format.extent415-422
dc.identifierhttp://dx.doi.org/10.1097/00002030-199704000-00003
dc.identifier.citationAids. London: Rapid Science Publishers, v. 11, n. 4, p. 415-422, 1997.
dc.identifier.doi10.1097/00002030-199704000-00003
dc.identifier.issn0269-9370
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/25705
dc.identifier.wosWOS:A1997WN61800003
dc.language.isoeng
dc.publisherRapid Science Publishers
dc.relation.ispartofAids
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectdisease progressionen
dc.subjectheterogeneityen
dc.subjectmolecular biologyen
dc.titleDivergence of HIV-1 quasispecies in an epidemiologic clusteren
dc.typeinfo:eu-repo/semantics/article
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