Molecular characterization of hepatitis C virus in end-stage renal disease patients under hemodialysis

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dc.citation.issue3
dc.citation.volume90
dc.contributor.authorda Silva, Rafael Alves [UNIFESP]
dc.contributor.authorTodao, Jardelina de Souza [UNIFESP]
dc.contributor.authorKamitani, Fernando Luiz
dc.contributor.authorBenedito Silva, Antonio Eduardo [UNIFESP]
dc.contributor.authorde Carvalho-Filho, Roberto Jose [UNIFESP]
dc.contributor.authorCardoso Gomes Ferraz, Maria Lucia [UNIFESP]
dc.contributor.authorVicente Guedes de Carvalho, Isabel Maria [UNIFESP]
dc.coverageHoboken
dc.date.accessioned2020-07-08T13:09:37Z
dc.date.available2020-07-08T13:09:37Z
dc.date.issued2018
dc.description.abstractNew direct-acting antiviral (DAA) agents are in development or already approved for the treatment of chronic hepatitis C virus (HCV) infection. The effectiveness of these drugs is related to the previous existence of resistant variants. Certain clinical conditions can allow changes in immunological characteristics of the host and even modify genetic features of viral populations. The aim of this study was to perform HCV molecular characterization from samples of end-stage renal disease patients on hemodialysis (ESRD-HD). Nested PCR and Sanger sequencing were used to obtain genetic information from the NS5B partial region of a cohort composed by 86 treatment-naive patients. Genomic sequences from the Los Alamos databank were employed for comparative analysis. Bioinformatics methodologies such as phylogenetic reconstructions, informational entropy, and mutation analysis were used to analyze datasets separated by geographical location, HCV genotype, and renal function status. ESRD-HD patients presented HCV genotypes 1a (n=18), 1b (n=16), 2a (n=2), 2b (n=2), and 3a (n=4). Control subjects were infected with genotypes 1a (n=11), 1b (n=21), 2b (n=4), and 3a (n=8). Dataset phylogenetic reconstruction separated HCV subtype 1a into two distinct clades. The entropy analysis from the ESRD-HD group revealed two amino acid positions related to an epitope for cytotoxic T lymphocytes and T helper cells. Genotype 1a was found to be more diverse than subtype 1b. Also, genotype 1a ERSD-HD patients had a higher mean of amino acids changes in comparison to control group patients. The identification of specific mutations on epitopes and high genetic diversity within the NS5B HCV partial protein in hemodialysis patients can relate to host immunological features and geographical distribution patterns. This genetic diversity can affect directly the new DAA's resistance mechanisms.en
dc.description.affiliationUniv Fed Sao Paulo, Lab Hepatol Mol Aplicada LHeMA, UNIFESP, Dept Gastroenterol,Unidade Hepatol, Sao Paulo, Brazil
dc.description.affiliationInst Butantan, Lab Parasitol, Ave Vital Brasil 1500, BR-05503900 Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Gastroenterol, Unidade Hepatol, UNIFESP, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Lab Hepatol Mol Aplicada LHeMA, UNIFESP, Dept Gastroenterol,Unidade Hepatol, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Gastroenterol, Unidade Hepatol, UNIFESP, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) [88881.132760/2016-01]
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2012/18168-2]
dc.description.sponsorshipIDCAPES: 8881.132760/2016-01
dc.description.sponsorshipIDFAPESP: 2012/18168-2
dc.format.extent537-544
dc.identifierhttp://dx.doi.org/10.1002/jmv.24976
dc.identifier.citationJournal Of Medical Virology. Hoboken, v. 90, n. 3, p. 537-544, 2018.
dc.identifier.doi10.1002/jmv.24976
dc.identifier.fileWOS000419510600021.pdf
dc.identifier.issn0146-6615
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54101
dc.identifier.wosWOS:000419510600021
dc.language.isoeng
dc.publisherWiley
dc.relation.ispartofJournal Of Medical Virology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectevolutionen
dc.subjectgenetic variabilityen
dc.subjectgeneticsen
dc.subjecthepatitis C virusen
dc.subjectmutationen
dc.subjectvirus classificationen
dc.titleMolecular characterization of hepatitis C virus in end-stage renal disease patients under hemodialysisen
dc.typeinfo:eu-repo/semantics/article
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