Early nuclear alterations and immunohistochemical expression of Ki-67, Erb-B2, vascular endothelial growth factor (VEGF), transforming growth factor (TGF-BETA 1) and integrine-linked kinase (ILK) two days after tamoxifen in breast carcinoma

Página do item simplificado
dc.contributor.authorMorena, Ana Maria Lira [UNIFESP]
dc.contributor.authorOshima, Celina Tizuko Fujiyama [UNIFESP]
dc.contributor.authorGebrim, Luiz Henrique [UNIFESP]
dc.contributor.authorEgami, Mizue Imoto [UNIFESP]
dc.contributor.authorSilva, Maria Regina Regis da [UNIFESP]
dc.contributor.authorSegreto, Roberto Araujo [UNIFESP]
dc.contributor.authorGiannotti Filho, Osvaldo [UNIFESP]
dc.contributor.authorTeixeira, Vicente de Paulo Castro [UNIFESP]
dc.contributor.authorSegreto, Helena Regina Comodo [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionOncoctr Fdn Sao Paulo
dc.date.accessioned2018-06-18T11:15:14Z
dc.date.available2018-06-18T11:15:14Z
dc.date.issued2004-01-01
dc.description.abstractThe purpose of the present study was to evaluate breast carcinoma samples before and two days after treatment with tamoxifen in order to analyse early histopathological alterations - particularly nuclear alterations - as well as immunohistochemical expression of Ki-67, Erb-B2, VEGF, TGf-beta 1 and ILK proteins. Twenty one cases of invasive ductal and lobular breast carcinoma were studied. Patients were submitted to biopsy of the lesion and, after confirmation of the diagnosis, they received 20 mg of tamoxifen a day, beginning two days before surgery. The samples obtained during biopsy and after surgery were stained with HE for histopathological diagnosis. Estrogen receptor was positive in IS cases and negative in 3. The immunohistochemical method was applied for the detection of Ki-67, Erb-B2, protein, vascular endothelial growth factor (VEGF), transforming growth factor beta (TGF-beta 1) and integrin linked kinase (ILK).Two days after tamoxifen treatment, the following results were observed: 1) decrease in the cell volume, chomatine condensation, nucleoli less evident and clearly defined nuclear limits; 2) significant reduction in the expression of Erb-B2 protein and significant increase in the expression of TGF-beta 1 protein; 3) expression of others proteins (Ki-67, VEGF and ILK) was not altered during the indicated time frame.Our results suggest that analyzing nuclear alterations and expression of Erb-B2 and TGF-beta 1 proteins would be useful to assess the initial response to tamoxifen.en
dc.description.affiliationUniv Fed Sao Paulo UNIFESP, Div Radiotherapy, Dept Med, Sao Paulo, Brazil
dc.description.affiliationUNIFESP, Dept Pathol, Sao Paulo, Brazil
dc.description.affiliationOncoctr Fdn Sao Paulo, Sao Paulo, Brazil
dc.description.affiliationUNIFESP, Discipline Mastol, Dept Tocogynecol, Sao Paulo, Brazil
dc.description.affiliationUNIFESP, Dept Morphol, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo UNIFESP, Div Radiotherapy, Dept Med, Sao Paulo, Brazil
dc.description.affiliationUnifespUNIFESP, Dept Pathol, Sao Paulo, Brazil
dc.description.affiliationUnifespUNIFESP, Discipline Mastol, Dept Tocogynecol, Sao Paulo, Brazil
dc.description.affiliationUnifespUNIFESP, Dept Morphol, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent481-486
dc.identifierhttp://www.elis.sk/index.php?page=shop.product_details&flypage=flypage-ask.tpl&product_id=846&category_id=32&option=com_virtuemart&Itemid=11&vmcchk=1&Itemid=11
dc.identifier.citationNeoplasma. Brastislava: Veda, Slovak Academy Sciences, v. 51, n. 6, p. 481-486, 2004.
dc.identifier.issn0028-2685
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/44983
dc.identifier.wosWOS:000226042900012
dc.language.isoeng
dc.publisherVeda, Slovak Academy Sciences
dc.relation.ispartofNeoplasma
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectbreast canceren
dc.subjecttamoxifenen
dc.subjectimmunohistochemistryen
dc.subjecttumor markersen
dc.subjectErb-B2en
dc.subjectTGF-beta 1en
dc.titleEarly nuclear alterations and immunohistochemical expression of Ki-67, Erb-B2, vascular endothelial growth factor (VEGF), transforming growth factor (TGF-BETA 1) and integrine-linked kinase (ILK) two days after tamoxifen in breast carcinomaen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
EPM - Artigos