Lysosomal integral membrane protein 2 (LIMP-2) restricts the invasion of Trypanosoma cruzi extracellular amastigotes through the activity of the lysosomal enzyme beta-glucocerebrosidase

Página do item simplificado
dc.contributor.authorGoncalves, Viviane Martinelli [UNIFESP]
dc.contributor.authorD'Almeida, Vania [UNIFESP]
dc.contributor.authorMueller, Karen Barbosa [UNIFESP]
dc.contributor.authorReal, Fernando [UNIFESP]
dc.contributor.authorMortara, Renato Arruda [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:35:23Z
dc.date.available2016-01-24T14:35:23Z
dc.date.issued2014-03-01
dc.description.abstractLysosomal integral membrane protein 2 (LDM1P-2, SCARB2) is directly linked to beta-glucocerebrosidase enzyme (beta GC) and mediates the transport of this enzyme from the Golgi complex to lysosomes. Active beta GC cleaves the beta-glycosidic linkages of glucosylceramide, an intermediate in the metabolism of sphingoglycolipids, generating ceramide. in this study we used mouse embryonic fibroblasts (MEFs) deficient for LIMP-2 and observed that these cells were more susceptible to infection by extracellular amastigotes of the protozoan parasite Trypanosoma cruzi when compared to wild-type (WT) fibroblasts. the absence of LIMP-2 decreases the activity of beta GC measured in fibroblast extracts. Replacement of beta GC enzyme in LIMP-2 deficient fibroblasts restores the infectivity indices to those of WT cells in T cruzti invasion assays. Considering the participation of beta GC in the production of host cell ceramide, we propose that T cruzi extracellular amastigotes are more invasive to cells deficient in this membrane component. These results contribute to our understanding of the role of host cell lysosomal components in T cruzi invasion. (C) 2013 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Psychobiol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Pediat, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Psychobiol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Pediat, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIDFAPESP: 10/19335-4
dc.format.extent253-260
dc.identifierhttp://dx.doi.org/10.1016/j.micinf.2013.11.004
dc.identifier.citationMicrobes and Infection. Amsterdam: Elsevier B.V., v. 16, n. 3, p. 253-260, 2014.
dc.identifier.doi10.1016/j.micinf.2013.11.004
dc.identifier.issn1286-4579
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/37496
dc.identifier.wosWOS:000347864600010
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofMicrobes and Infection
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectLIMP-2en
dc.subjectbeta-Glucocerebrosidaseen
dc.subjectTrypanosoma cruzien
dc.subjectExtracellular amastigotesen
dc.titleLysosomal integral membrane protein 2 (LIMP-2) restricts the invasion of Trypanosoma cruzi extracellular amastigotes through the activity of the lysosomal enzyme beta-glucocerebrosidaseen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
EPM - Artigos