Detection of herpes simplex-1 and-2 and varicella zoster virus by quantitative real-time polymerase chain reaction in corneas from patients with bacterial keratitis

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dc.citation.issue2
dc.citation.volume80
dc.contributor.authorNascimento, Heloisa [UNIFESP]
dc.contributor.authorWatanabe, Aripuanã Sakurada Aranha [UNIFESP]
dc.contributor.authorVieira, Ana Carolina Cabreira [UNIFESP]
dc.contributor.authorPellegrini, Andrea Lucia Torres Amorim [UNIFESP]
dc.contributor.authorYu, Maria Ceclia [UNIFESP]
dc.contributor.authorBispo, Paulo José Martins [UNIFESP]
dc.contributor.authorGranato, Celso Francisco Hernandes [UNIFESP]
dc.contributor.authorHofling-Lima, Ana Luisa
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.coverageSao Paulo
dc.date.accessioned2020-07-17T14:02:42Z
dc.date.available2020-07-17T14:02:42Z
dc.date.issued2017
dc.description.abstractObjective: Bacterial keratitis occurs worldwide, and despite recent developments, it remains a potentially blinding condition. This study assesses the presence of herpes simplex virus (HSV-1 and -2) and varicella zoster virus (VZV) by quantitative real-time polymerase chain reaction (qPCR) in corneal scrapings from patients with bacterial keratitis. Methods: A total of 65 patients with clinical diagnoses of infectious corneal ulcers prospectively underwent clinical eye examinations. Corneal scrapings were investigated by Gram staining, Giemsa staining, culture, and qPCR (the study group). Risk factors and epidemiological data were recorded. The control group comprising 25 eyes with typical herpes dendritic keratitis was also analyzed by qPCR. Results: From the study group (n=65), nine patients (13.8%) had negative smears, cultures, and qPCR findings. Fifty-six (86.2%) patients had positive cultures: 51 for bacteria, 4 for fungi, and 1 for amoebae. Of the patients who had positive bacterial cultures, qPCR identified 10 patients who were also positive for virus: one for VZV and nine for HSV-1. Of the 25 patients in the control group, 21 tested positive for HSV-1 by qPCR analysis. Conclusions: Herpes may be present in patients with bacterial corneal ulcers, and qPCR may be useful in its detection.en
dc.description.affiliationUniv Fed Sao Paulo UNIFESP, EPM, Dept Ophthalmol & Visual Sci, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo UNIFESP, EPM, Dept Med, Infectol Discipline, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo UNIFESP, EPM, Dept Ophthalmol & Visual Sci, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo UNIFESP, EPM, Dept Med, Infectol Discipline, Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipSao Paulo State Research Foundation (FAPESP)
dc.format.extent84-87
dc.identifierhttps://www.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492017000200084&lng=es&nrm=iso&tlng=es
dc.identifier.citationArquivos Brasileiros De Oftalmologia. Sao Paulo, v. 80, n. 2, p. 84-87, 2017.
dc.identifier.doi10.5935/0004-2749.20170021
dc.identifier.fileS0004-27492017000200084.pdf
dc.identifier.issn0004-2749
dc.identifier.scieloS0004-27492017000200084
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54954
dc.identifier.wosWOS:000403248600005
dc.language.isoeng
dc.publisherConsel Brasil Oftalmologia
dc.relation.ispartofArquivos Brasileiros De Oftalmologia
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectHerpes simplexen
dc.subjectHerpesviridaeen
dc.subjectKeratitis, herpeticen
dc.subjectPolymerase chain reactionen
dc.titleDetection of herpes simplex-1 and-2 and varicella zoster virus by quantitative real-time polymerase chain reaction in corneas from patients with bacterial keratitisen
dc.typeinfo:eu-repo/semantics/article
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