High expression of TIM-3 mRNA in urinary cells from kidney transplant recipients with acute rejection

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dc.contributor.authorRenesto, P. G.
dc.contributor.authorPonciano, V. C.
dc.contributor.authorCenedeze, Marcos Antonio [UNIFESP]
dc.contributor.authorCamara, N. O. Saraiva
dc.contributor.authorPacheco-Silva, Alvaro [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T13:48:45Z
dc.date.available2016-01-24T13:48:45Z
dc.date.issued2007-06-01
dc.description.abstractTIM-3 is a recently described molecule specifically expressed on Th1 differentiated T cells. We explored the usefulness of urinary mRNA profiles in the diagnosis of renal acute rejection (AR). Sixty urinary samples from renal transplant recipients simultaneously collected to allograft biopsy, (AR = 30 and No-AR =30), and 12 urinary samples from stable renal transplants were analyzed. Urinary mRNA encoding for TIM-3 and IFN-gamma were quantified using real time RT-PCR. TIM-3 mRNA was highly expressed in AR (559.19 +/- 644.41) compared to No-AR (3.78 +/- 7.20), and stable transplants (0.54 +/- 0.76), p < 0.001. To a lesser degree, IFN-gamma mRNA transcripts were also increased in AR (50.40 +/- 38.71), compared with No-AR (4.69 +/- 12.62), and stable transplants (0.38 +/- 0.44) p < 0.001. the highest expression of TIM-3 in AR makes it a promising noninvasive test for its diagnosis.en
dc.description.affiliationUniversidade Federal de São Paulo, Div Nephrol, Clin & Expt Immunol Lab, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Lab Transplant Immunobiol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Div Nephrol, Clin & Expt Immunol Lab, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent1661-1665
dc.identifierhttp://dx.doi.org/10.1111/j.1600-6143.2007.01795.x
dc.identifier.citationAmerican Journal of Transplantation. Oxford: Blackwell Publishing, v. 7, n. 6, p. 1661-1665, 2007.
dc.identifier.doi10.1111/j.1600-6143.2007.01795.x
dc.identifier.issn1600-6135
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/29771
dc.identifier.wosWOS:000246576700030
dc.language.isoeng
dc.publisherBlackwell Publishing
dc.relation.ispartofAmerican Journal of Transplantation
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectgraft rejectionen
dc.subjectgraft survivalen
dc.subjectimmune monitoringen
dc.subjectimmune rejectionen
dc.subjectrenal transplantationen
dc.subjectTIM-3en
dc.titleHigh expression of TIM-3 mRNA in urinary cells from kidney transplant recipients with acute rejectionen
dc.typeinfo:eu-repo/semantics/article
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