Dimorphic expression of cerebrosides in the mycopathogen Sporothrix schenckii

dc.contributor.authorToledo, Marcos S. [UNIFESP]
dc.contributor.authorLevery, Steven B.
dc.contributor.authorStraus, Anita H. [UNIFESP]
dc.contributor.authorTakahashi, Helio K. [UNIFESP]
dc.contributor.institutionUniv Georgia
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2018-06-15T18:07:20Z
dc.date.available2018-06-15T18:07:20Z
dc.date.issued2000-05-01
dc.description.abstractMajor neutral glycosphingolipid components were extracted from Sporothrix schenckii, a dimorphic fungus exhibiting a hyphal saprophytic phase and a yeast parasitic phase responsible for chronic mycotic infections in mammalian hosts. These components, one from the mycelial form and two from the yeast form, were purified and their structures were elucidated by H-1 nuclear magnetic resonance (NMR) spectroscopy, electrospray ionization mass spectrometry (ESI-MS), and tandem ESI-MS/MS, All three were characterized as cerebrosides (monohexosylceramides) containing (4E, 8E)-9-methyl-4,8-sphingadienine as the long-chain base attached to N-2'-hydroxyoctadecanoate and N2'-hydroxy-(E)-Delta(3)-octadecenoate as the fatty acyl components. However, while the mycelial form expressed only beta-glucopyranosylceramide, the yeast form expressed both beta-gluco- and beta-galactopyranosylceramides in approximately equal amounts. In addition, while the glucosylceramides of both mycelial and yeast forms had similar proportions of saturated and (E)-Delta(3) unsaturated 2-hydroxy fatty acid, the galactocerebroside of the yeast form had significantly higher levels of (E)Delta(3) unsaturation. The differences in cerebroside hexose structure represent a novel type of glycosphingolipid dimorphism not previously reported in fungi, Possible implications of these findings with respect to regulation of morphological transitions in S, schenckii and other dimorphic fungi are discussed. Dimorphic expression of cerebrosides in the mycopathogen Sporothrix schenckii.en
dc.description.affiliationUniv Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA
dc.description.affiliationUniv Georgia, Complex Carbohydrate Res Ctr, Athens, GA 30602 USA
dc.description.affiliationUniv Fed Sao Paulo, Escola Paulista Med, Dept Biochem, BR-04023900 Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Escola Paulista Med, Dept Biochem, BR-04023900 Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.format.extent797-806
dc.identifierhttp://www.jlr.org/content/41/5/797.abstract
dc.identifier.citationJournal Of Lipid Research. Bethesda: Lipid Research Inc, v. 41, n. 5, p. 797-806, 2000.
dc.identifier.issn0022-2275
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/44472
dc.identifier.wosWOS:000087054200014
dc.language.isoeng
dc.publisherLipid Research Inc
dc.relation.ispartofJournal Of Lipid Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectelectrospray ionizationen
dc.subjectmass spectrometryen
dc.subjectESI-MSen
dc.subjectnuclear magnetic resonance spectroscopyen
dc.subjectNMRen
dc.subjectcollision-induced dissociationen
dc.subjecttandem mass spectrometryen
dc.subjectfungusen
dc.subjectyeasten
dc.subjectmycosisen
dc.titleDimorphic expression of cerebrosides in the mycopathogen Sporothrix schenckiien
dc.typeinfo:eu-repo/semantics/article
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