Dimorphic expression of cerebrosides in the mycopathogen Sporothrix schenckii
dc.contributor.author | Toledo, Marcos S. [UNIFESP] | |
dc.contributor.author | Levery, Steven B. | |
dc.contributor.author | Straus, Anita H. [UNIFESP] | |
dc.contributor.author | Takahashi, Helio K. [UNIFESP] | |
dc.contributor.institution | Univ Georgia | |
dc.contributor.institution | Universidade Federal de São Paulo (UNIFESP) | |
dc.date.accessioned | 2018-06-15T18:07:20Z | |
dc.date.available | 2018-06-15T18:07:20Z | |
dc.date.issued | 2000-05-01 | |
dc.description.abstract | Major neutral glycosphingolipid components were extracted from Sporothrix schenckii, a dimorphic fungus exhibiting a hyphal saprophytic phase and a yeast parasitic phase responsible for chronic mycotic infections in mammalian hosts. These components, one from the mycelial form and two from the yeast form, were purified and their structures were elucidated by H-1 nuclear magnetic resonance (NMR) spectroscopy, electrospray ionization mass spectrometry (ESI-MS), and tandem ESI-MS/MS, All three were characterized as cerebrosides (monohexosylceramides) containing (4E, 8E)-9-methyl-4,8-sphingadienine as the long-chain base attached to N-2'-hydroxyoctadecanoate and N2'-hydroxy-(E)-Delta(3)-octadecenoate as the fatty acyl components. However, while the mycelial form expressed only beta-glucopyranosylceramide, the yeast form expressed both beta-gluco- and beta-galactopyranosylceramides in approximately equal amounts. In addition, while the glucosylceramides of both mycelial and yeast forms had similar proportions of saturated and (E)-Delta(3) unsaturated 2-hydroxy fatty acid, the galactocerebroside of the yeast form had significantly higher levels of (E)Delta(3) unsaturation. The differences in cerebroside hexose structure represent a novel type of glycosphingolipid dimorphism not previously reported in fungi, Possible implications of these findings with respect to regulation of morphological transitions in S, schenckii and other dimorphic fungi are discussed. Dimorphic expression of cerebrosides in the mycopathogen Sporothrix schenckii. | en |
dc.description.affiliation | Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA | |
dc.description.affiliation | Univ Georgia, Complex Carbohydrate Res Ctr, Athens, GA 30602 USA | |
dc.description.affiliation | Univ Fed Sao Paulo, Escola Paulista Med, Dept Biochem, BR-04023900 Sao Paulo, SP, Brazil | |
dc.description.affiliationUnifesp | Univ Fed Sao Paulo, Escola Paulista Med, Dept Biochem, BR-04023900 Sao Paulo, SP, Brazil | |
dc.description.source | Web of Science | |
dc.format.extent | 797-806 | |
dc.identifier | http://www.jlr.org/content/41/5/797.abstract | |
dc.identifier.citation | Journal Of Lipid Research. Bethesda: Lipid Research Inc, v. 41, n. 5, p. 797-806, 2000. | |
dc.identifier.issn | 0022-2275 | |
dc.identifier.uri | http://repositorio.unifesp.br/handle/11600/44472 | |
dc.identifier.wos | WOS:000087054200014 | |
dc.language.iso | eng | |
dc.publisher | Lipid Research Inc | |
dc.relation.ispartof | Journal Of Lipid Research | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | electrospray ionization | en |
dc.subject | mass spectrometry | en |
dc.subject | ESI-MS | en |
dc.subject | nuclear magnetic resonance spectroscopy | en |
dc.subject | NMR | en |
dc.subject | collision-induced dissociation | en |
dc.subject | tandem mass spectrometry | en |
dc.subject | fungus | en |
dc.subject | yeast | en |
dc.subject | mycosis | en |
dc.title | Dimorphic expression of cerebrosides in the mycopathogen Sporothrix schenckii | en |
dc.type | info:eu-repo/semantics/article |