Association between the p53 arginine/arginine homozygous genotype at codon 72 and human papillomavirus E6/E7 mRNA expression

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dc.citation.issue3
dc.citation.volume21
dc.contributor.authorSilva Chuery, Ana Carolina [UNIFESP]
dc.contributor.authorCotrim Guerreiro da Silva, Ismael Dale [UNIFESP]
dc.contributor.authorLascasas Ribalta, Julisa Chamorro [UNIFESP]
dc.contributor.authorde Gois Speck, Neila Maria [UNIFESP]
dc.coverageRio De Janeiro
dc.date.accessioned2020-07-13T11:53:19Z
dc.date.available2020-07-13T11:53:19Z
dc.date.issued2017
dc.description.abstractObjective: To evaluate the association between p53 polymorphisms and human papillomavirus (HPV) E6/E7 mRNA expression. Methods: We analyzed 175 cervical samples from women aged 16-69 years old who were tested for HPV E6/E7 mRNA expression (NucliSENS (R) EasyQ (R) HPV). The samples were divided into three groups: positive (n = 75) those with positive HPV E6/E7 mRNA expression and positive high-risk HPV Hybrid Capture (HR-HC) testen
dc.description.abstractnegative (n = 52) those with negative HPV E6/E7 mRNA expression and positive HR-HCen
dc.description.abstractand control (n = 48) those with negative HPV E6/E7 mRNA expression and negative HR-HC. The p53 polymorphisms at codons 11, 72, and 248 were evaluated through polymerase chain reaction-restriction fragment length polymorphism( PCR-RFLP). Results: The frequency of the arginine/arginine homozygous genotype at codon 72 was significantly higher in the positive (49.3%) than in the negative (32.7%) and control groups (20.8%, p = 0.002*). The frequency of the arginine allele was also significantly higher in the positive (67.3%) than in the negative (53.8%) and control groups (38.5%, p < 0.001*). The arginine/arginine homozygous genotype was significantly associated with positive HPV E6/E7 mRNA expression (positive group) compared with negative and control groups (odds ratio: 2.633en
dc.description.abstract95% CI, 1.399-4.954, p = 0.003). The frequency of arginine/arginine homozygous genotype at codon 72 remained significantly more frequent in the positive group of women aged >= 30 years than in the other two groups. Conclusion: The presence of the p53 arginine/arginine homozygous genotype at codon 72 was significantly associated with the positive HPV E6/E7 mRNA expression. (C) 2017 Sociedade Brasileira de Infectologia. Published by Elsevier Editora Ltda.en
dc.description.affiliationUniv Fed Sao Paulo Unifesp, Dept Ginecol, Nucleo Prevencao Doencas Ginecol, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo Unifesp, Dept Ginecol, Escola Paulista Med, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo Unifesp, Dept Ginecol, Nucleo Prevencao Doencas Ginecol, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo Unifesp, Dept Ginecol, Escola Paulista Med, Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.format.extent248-254
dc.identifierhttp://dx.doi.org/10.1016/j.bjid.2017.03.002
dc.identifier.citationBrazilian Journal Of Infectious Diseases. Rio De Janeiro, v. 21, n. 3, p. 248-254, 2017.
dc.identifier.doi10.1016/j.bjid.2017.03.002
dc.identifier.issn1413-8670
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54537
dc.identifier.wosWOS:000404453600006
dc.language.isoeng
dc.publisherElsevier Brazil
dc.relation.ispartofBrazilian Journal Of Infectious Diseases
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectHuman papillomavirusen
dc.subjectOncoproteinsen
dc.subjectGenetic polymorphismsen
dc.subjectp53 geneen
dc.subjectArginineaen
dc.titleAssociation between the p53 arginine/arginine homozygous genotype at codon 72 and human papillomavirus E6/E7 mRNA expressionen
dc.typeinfo:eu-repo/semantics/article
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