Inhibitory effect of the Pseudobrickellia brasiliensis (Spreng) RM King & H. Rob. aqueous extract on human lymphocyte proliferation and IFN-gamma and TNF-alpha production in vitro

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dc.citation.issue8
dc.citation.volume50
dc.contributor.authorAlmeida, V. G.
dc.contributor.authorAvelar-Freitas, B. A.
dc.contributor.authorSantos, M. G.
dc.contributor.authorCosta, L. A.
dc.contributor.authorSilva, T. J.
dc.contributor.authorPereira, W. F.
dc.contributor.authorAmorim, M. L. L.
dc.contributor.authorGrael, C. F. F.
dc.contributor.authorGregorio, Luiz Elídio [UNIFESP]
dc.contributor.authorRocha-Vieira, E.
dc.contributor.authorBrito-Melo, G. E. A.
dc.coverageSao Paulo
dc.date.accessioned2020-07-31T12:46:29Z
dc.date.available2020-07-31T12:46:29Z
dc.date.issued2017
dc.description.abstractPseudobrickellia brasiliensis (Asteraceae) is a plant commonly known as arnica-do-campo and belongs to the native flora of the Brazilian Cerrado. The alcoholic extract of the plant has been used as an anti-inflammatory agent in folk medicine, but the biological mechanism of action has not been elucidated. The present study evaluated the composition of P. brasiliensis aqueous extract and its effects on pro-inflammatory cytokine production and lymphocyte proliferation. The extracts were prepared by sequential maceration of P. brasiliensis leaves in ethanol, ethyl acetate, and water. Extract cytotoxicity was evaluated by trypan blue exclusion assay, and apoptosis and necrosis were measured by staining with annexin V-FITC and propidium iodide. The ethanolic (ETA) and acetate (ACE) extracts showed cytotoxic effects. The aqueous extract (AQU) was not cytotoxic. Peripheral blood mononuclear cells stimulated with phorbol myristate acetate and ionomycin and treated with AQU (100 mu g/mL) showed reduced interferon (IFN)-gamma and tumor necrosis factor (TNF)-alpha expression. AQU also inhibited lymphocyte proliferative response after nonspecific stimulation with phytohemagglutinin. The aqueous extract was analyzed by liquid chromatography coupled with photodiode array detection and mass spectrometry. Quinic acid and its derivatives 5-caffeoylquinic acid and 3,5dicaffeoylquinic acid, as well as the flavonoids luteolin and luteolin dihexoside, were detected. All these compounds are known to exhibit anti-inflammatory activity. Taken together, these findings demonstrate that P. brasiliensis aqueous extract can inhibit the pro-inflammatory cytokine production and proliferative response of lymphocytes. These effects may be related to the presence of chemical substances with anti-inflammatory actions previously reported in scientific literature.en
dc.description.affiliationUniv Fed Vales Jequitinhonha & Mucuri, Programa Multictr Posgrad Ciencias Fisiol, Diamantina, MG, Brazil
dc.description.affiliationUniv Fed Vales Jequitinhonha & Mucuri, Ctr Integrado Posgrad & Pesquisa Saude Vale Jequi, Lab Imunol, Diamantina, MG, Brazil
dc.description.affiliationUniv Fed Vales Jequitinhonha & Mucuri, Dept Farm, Lab Farmacognosia, Diamantina, MG, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Ciencias Farmaceut, Lab Insumos Nat & Sintet, Diadema, SP, Brazil
dc.description.affiliationUnifespLaboratório de Insumos Naturais e Sintéticos, Departamento de Ciências Farmacêuticas, Universidade Federal de São Paulo, Diadema, SP, Brasil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1590/1414-431X20175163
dc.identifier.citationBrazilian Journal Of Medical And Biological Research. Sao Paulo, v. 50, n. 8, p. -, 2017.
dc.identifier.doi10.1590/1414-431X20175163
dc.identifier.fileS0100-879X2017000800603.pdf
dc.identifier.issn0100-879X
dc.identifier.scieloS0100-879X2017000800603
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56256
dc.identifier.wosWOS:000405405900001
dc.language.isoeng
dc.publisherAssoc Bras Divulg Cientifica
dc.relation.ispartofBrazilian Journal Of Medical And Biological Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCytokinesen
dc.subjectLymphocyte proliferationen
dc.subjectLuteolinen
dc.subjectMedicinal planten
dc.subjectPseudobrickellia brasiliensisen
dc.subjectQuinic aciden
dc.titleInhibitory effect of the Pseudobrickellia brasiliensis (Spreng) RM King & H. Rob. aqueous extract on human lymphocyte proliferation and IFN-gamma and TNF-alpha production in vitroen
dc.typeinfo:eu-repo/semantics/article
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