Single nucleotide polymorphisms of the heat shock protein 90 gene in varicocele-associated infertility

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dc.contributor.authorHassun Filho, Pericles A. [UNIFESP]
dc.contributor.authorCedenho, Agnaldo Pereira [UNIFESP]
dc.contributor.authorLima, Samira B. [UNIFESP]
dc.contributor.authorOrtiz, Valdemar [UNIFESP]
dc.contributor.authorSrougi, Miguel [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2015-06-14T13:31:37Z
dc.date.available2015-06-14T13:31:37Z
dc.date.issued2005-06-01
dc.description.abstractPURPOSE: Varicoceles are associated with impaired testicular function and male infertility, but the molecular mechanisms by which fertility is affected have not been satisfactorily explained. Spermatogenesis might be affected by increased scrotal temperature, such as that caused by varicocele. HSP90 is a molecular chaperone expressed in germ cells and is related to spermatogenesis, motility, and both heat and oxidative stress. Possible correlations between coding single region nucleotide polymorphisms (cSNPs) in the HSP90 gene in patients with varicocele associated with infertility were analyzed, and polymorphisms in these exons were characterized through DNA sequencing. MATERIALS AND METHODS: PCR-SSCP and DNA sequencing were used to search for mutations in 18 infertile patients with varicocele, 11 patients with idiopathic infertility and 12 fertile men. DNA was extracted from leucocytes for PCR amplification and SSCP analysis. DNA from samples with an altered band pattern in the SSCP was then sequenced to search for polymorphisms. RESULTS: Three silent polymorphisms that do not lead to amino acid substitutions were identified. CONCLUSION: Mutations in the HSP90 gene do not appear to be a common cause of male factor infertility. The low incidence of gene variation, or SNPs, in infertile men demonstrates that this gene is highly conserved and thus confirms its key role in spermatogenesis and response to heat stress.en
dc.description.affiliationFederal University of São Paulo Division of Urology Department of Surgery
dc.description.affiliationUnifespUNIFESP, Division of Urology Department of Surgery
dc.description.sourceSciELO
dc.format.extent236-244
dc.identifierhttp://dx.doi.org/10.1590/S1677-55382005000300007
dc.identifier.citationInternational braz j urol. Sociedade Brasileira de Urologia, v. 31, n. 3, p. 236-244, 2005.
dc.identifier.doi10.1590/S1677-55382005000300007
dc.identifier.fileS1677-55382005000300007.pdf
dc.identifier.issn1677-5538
dc.identifier.scieloS1677-55382005000300007
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/2549
dc.language.isoeng
dc.publisherSociedade Brasileira de Urologia
dc.relation.ispartofInternational braz j urol
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectvaricoceleen
dc.subjectheat stressen
dc.subjectHeat-Shock Protein 90en
dc.subjectspermatozoaen
dc.subjectinfertilityen
dc.subjectgenetic polymorphismen
dc.titleSingle nucleotide polymorphisms of the heat shock protein 90 gene in varicocele-associated infertilityen
dc.typeinfo:eu-repo/semantics/article
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