Fibroblast and prostate tumor cell cross-talk: Fibroblast differentiation, TGF-beta, and extracellular matrix down-regulation

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dc.contributor.authorCoulson-Thomas, Vivien Jane [UNIFESP]
dc.contributor.authorGesteira, Tarsis F.
dc.contributor.authorCoulson-Thomas, Yvette May [UNIFESP]
dc.contributor.authorVicente, Carolina M.
dc.contributor.authorTersariol, Ivarne L. S.
dc.contributor.authorNader, Helena B.
dc.contributor.authorToma, Leny
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:05:41Z
dc.date.available2016-01-24T14:05:41Z
dc.date.issued2010-11-15
dc.description.abstractGrowth and survival of tumors at a site of metastasis involve interactions with stromal cells in the surrounding environment. Stromal cells aid tumor cell growth by producing cytokines as well as by modifying the environment surrounding the tumor through modulation of the extracellular matrix (ECM). Small leucine-rich proteoglycans (SLRPs) are biologically active components of the ECM which can be altered in the stroma surrounding tumors. the influence tumor cells have on stromal cells has been well elucidated. However, little is understood about the effect metastatic cancer cells have on the cell biology and behavior of the local stromal cells. Our data reveal a significant downregulation in the expression of ECM components such as collagens I, II, III, and IV, and the SLRPs, decorin, biglycan, lumican, and fibromodulin in stromal cells when grown in the presence of two metastatic prostate cancer cell lines PO and DU145. Interestingly, TGF-beta down-regulation was observed in stromal cells, as well as actin depolymerization and increased vimentin and alpha 5 beta 1 integrin expression. MT1-MMP expression was upregulated and localized in stromal cell protrusions which extended into the ECM. Moreover, enhanced stromal cell migration was observed after crosstalk with metastatic prostate tumor cells. Xenografting metastatic prostate cancer cells together with activated stromal cells led to increased tumorigenicity of the prostate cancer cells. Our findings suggest that metastatic prostate cancer cells create a metastatic niche by altering the phenotype of local stromal cells, leading to changes in the ECM. (C) 2010 Elsevier Inc. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Bioquim, Escola Paulista Med, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Bioquim, Escola Paulista Med, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIDFAPESP: 2007/59801-1
dc.format.extent3207-3226
dc.identifierhttp://dx.doi.org/10.1016/j.yexcr.2010.08.005
dc.identifier.citationExperimental Cell Research. San Diego: Elsevier Inc, v. 316, n. 19, p. 3207-3226, 2010.
dc.identifier.doi10.1016/j.yexcr.2010.08.005
dc.identifier.issn0014-4827
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/33085
dc.identifier.wosWOS:000285218600009
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofExperimental Cell Research
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectStromal reactionen
dc.subjectProteoglycansen
dc.subjectCollagensen
dc.subjectCytoskeletonen
dc.subjectMatrix metalloproteinasesen
dc.subjectVimentinen
dc.titleFibroblast and prostate tumor cell cross-talk: Fibroblast differentiation, TGF-beta, and extracellular matrix down-regulationen
dc.typeinfo:eu-repo/semantics/article
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