Rats with different thresholds for DMCM-induced clonic convulsions differ in the sleep-time of diazepam and [H-3]-Ro 15-4513 binding
| dc.contributor.author | Contó, Marcos Brandão [UNIFESP] | |
| dc.contributor.author | Hipólide, Débora Cristina [UNIFESP] | |
| dc.contributor.author | Barbosa de Carvalho, Jose Gilberto [UNIFESP] | |
| dc.contributor.author | Venditti, Marco Antonio Campana [UNIFESP] | |
| dc.contributor.institution | Universidade Federal de São Paulo (UNIFESP) | |
| dc.date.accessioned | 2016-01-24T14:17:52Z | |
| dc.date.available | 2016-01-24T14:17:52Z | |
| dc.date.issued | 2012-02-01 | |
| dc.description.abstract | The current study investigated the possible inherent relationship between convulsions and sleep involving the GABA(A)/benzodiazepine site complex. the aim of this study was to determine if rats with high (HTR) and low (LTR) thresholds for clonic convulsions induced by DMCM, a benzodiazepine inverse agonist, differ in the following aspects: (1) sensitivity to the hypnotic effects of the GABA(A) positive allosteric modulators diazepam, pentobarbital and ethanol and (2) in the binding of [H-3]-flunitrazepam, a benzodiazepine agonist, measured by autoradiography, and [H-3]-Ro 15-4513, a benzodiazepine partial inverse agonist, to membranes from discrete brain regions. the LTR subgroup presented a shorter diazepam-induced sleeping time compared to that of the HTR subgroup. Biochemical assays revealed that the LTR subgroup did not differ in [H-3]-flunitrazepam binding compared to the HTR subgroup. With respect to the binding of [H-3]-Ro 15-4513, the LTR subgroup had higher binding in the brainstem and lower binding in the striatum compared to the HTR subgroup. These results suggest that differences in the benzodiazepine site on the GABA(A) receptor may underlie the susceptibility to DMCM-induced convulsions and sensitivity to the hypnotic effect of diazepam. (C) 2011 Elsevier B.V. All rights reserved. | en |
| dc.description.affiliation | Universidade Federal de São Paulo, Escola Paulista Med UNIFESP EPM, Dept Psicobiol, BR-04023900 São Paulo, Brazil | |
| dc.description.affiliationUnifesp | Universidade Federal de São Paulo, Escola Paulista Med UNIFESP EPM, Dept Psicobiol, BR-04023900 São Paulo, Brazil | |
| dc.description.source | Web of Science | |
| dc.description.sponsorship | Associacao Fundo de Incentivo a Pesquisa (AFIP) | |
| dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | |
| dc.format.extent | 216-222 | |
| dc.identifier | http://dx.doi.org/10.1016/j.eplepsyres.2011.09.014 | |
| dc.identifier.citation | Epilepsy Research. Amsterdam: Elsevier B.V., v. 98, n. 2-3, p. 216-222, 2012. | |
| dc.identifier.doi | 10.1016/j.eplepsyres.2011.09.014 | |
| dc.identifier.issn | 0920-1211 | |
| dc.identifier.uri | http://repositorio.unifesp.br/handle/11600/34586 | |
| dc.identifier.wos | WOS:000301316200017 | |
| dc.language.iso | eng | |
| dc.publisher | Elsevier B.V. | |
| dc.relation.ispartof | Epilepsy Research | |
| dc.rights | info:eu-repo/semantics/restrictedAccess | |
| dc.rights.license | http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy | |
| dc.subject | [H-3]-flunitrazepam | en |
| dc.subject | [H-3]-Ro 15-4513 | en |
| dc.subject | DMCM | en |
| dc.subject | GABA(A) | en |
| dc.subject | Convulsions | en |
| dc.subject | Sleeping time | en |
| dc.title | Rats with different thresholds for DMCM-induced clonic convulsions differ in the sleep-time of diazepam and [H-3]-Ro 15-4513 binding | en |
| dc.type | info:eu-repo/semantics/article |