Combined fish oil and astaxanthin supplementation modulates rat lymphocyte function

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dc.contributor.authorOtton, Rosemari
dc.contributor.authorMarin, Douglas Popp
dc.contributor.authorBolin, Anaysa Paola
dc.contributor.authorSantos Macedo, Rita de Cassia
dc.contributor.authorCampoio, Thais Regina
dc.contributor.authorFineto, Claudio
dc.contributor.authorGuerra, Beatriz Alves
dc.contributor.authorLeite, Jose Roberto [UNIFESP]
dc.contributor.authorBarros, Marcelo Paes
dc.contributor.authorMattei, Rita [UNIFESP]
dc.contributor.institutionUniv Cruzeiro Sul
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:27:36Z
dc.date.available2016-01-24T14:27:36Z
dc.date.issued2012-09-01
dc.description.abstractHigher intakes of n-3 polyunsaturated fatty acids that are abundant in marine fishes have been long described as a good nutritional intervention with increasing clinical benefits to cardiovascular health, inflammation, mental, and neurodegenerative diseases. the present study was designed to investigate the effect of daily fish oil (FO-10 mg EPA/kg body weight (BW) and 7 mg DHA/kg BW) intake by oral gavage associated with the antioxidant astaxanthin (ASTA-1 mg/kg BW) on the redox metabolism and the functional properties of lymphocytes from rat lymph nodes.This study was conducted by measurements of lymphocyte proliferation capacity, ROS production [superoxide (O (2) (aEuro cent a') ) and hydrogen peroxide (H2O2)], nitric oxide (NOaEuro cent) generation, intracellular calcium release, oxidative damage to lipids and proteins, activities of major antioxidant enzymes, GSH/GSSG content, and cytokines release.After 45 days of FO + ASTA supplementation, the proliferation capacity of activated T- and B-lymphocytes was significantly diminished followed by lower levels of O (2) (aEuro cent a') , H2O2 and NOaEuro cent production, and increased activities of total/SOD, GR and GPx, and calcium release in cytosol. ASTA was able to prevent oxidative modification in cell structures through the suppression of the oxidative stress condition imposed by FO. l-selectin was increased by FO, and IL-1 beta was decreased only by ASTA supplementation.We can propose that association of ASTA with FO could be a good strategy to prevent oxidative stress induced by polyunsaturated fatty acids and also to potentiate immuno-modulatory effects of FO.en
dc.description.affiliationUniv Cruzeiro Sul, CBS, Postgrad Program, BR-03342000 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo UNIFESP, Dept Psicobiol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniv Cruzeiro Sul, Human Movement Sci Inst Phys Act & Sport Sci, Postgrad Program, BR-01506000 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo UNIFESP, Dept Psicobiol, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipUniversidade Cruzeiro do Sul
dc.description.sponsorshipDepartamento de Psicobiologia-UNIFESP/Associacao Fundo de Incentivo a Psicofarmacologia (AFIP)
dc.description.sponsorshipIDFAPESP: FAPESP 07/03334-6
dc.description.sponsorshipIDFAPESP: 2009/12342-8
dc.description.sponsorshipIDFAPESP: 2002/09405-9
dc.format.extent707-718
dc.identifierhttp://dx.doi.org/10.1007/s00394-011-0250-z
dc.identifier.citationEuropean Journal of Nutrition. Heidelberg: Springer Heidelberg, v. 51, n. 6, p. 707-718, 2012.
dc.identifier.doi10.1007/s00394-011-0250-z
dc.identifier.issn1436-6207
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/35205
dc.identifier.wosWOS:000307551400006
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofEuropean Journal of Nutrition
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.subjectAntioxidanten
dc.subjectAstaxanthinen
dc.subjectCarotenoiden
dc.subjectFish oilen
dc.subjectLeukocytesen
dc.subjectLymphocyteen
dc.subjectOxidative stressen
dc.subjectn-3 fatty acidsen
dc.titleCombined fish oil and astaxanthin supplementation modulates rat lymphocyte functionen
dc.typeinfo:eu-repo/semantics/article
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