Effects of Anti-IL-17 on Inflammation, Remodeling, and Oxidative Stress in an Experimental Model of Asthma Exacerbated by LPS

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dc.citation.volume8
dc.contributor.authorCamargo, Leandro do Nascimento
dc.contributor.authorRighetti, Renato Fraga
dc.contributor.authorAristoteles, Luciana Ritha de Cassia Rolim Barbosa
dc.contributor.authorSantos, Tabata Maruyama dos
dc.contributor.authorSouza, Flavia Castro Ribas de
dc.contributor.authorFukuzaki, Silvia
dc.contributor.authorCruz, Maysa Mariana [UNIFESP]
dc.contributor.authorAlonso-Vale, Maria Isabel Cardoso [UNIFESP]
dc.contributor.authorSaraiva-Romanholo, Beatriz Mangueira
dc.contributor.authorPrado, Carla Maximo [UNIFESP]
dc.contributor.authorMartins, Milton de Arruda
dc.contributor.authorLeick, Aparecida
dc.contributor.authorLopes Calvo Tiberio, Iolanda de Fatima
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.coverageLausanne
dc.date.accessioned2020-07-08T13:09:53Z
dc.date.available2020-07-08T13:09:53Z
dc.date.issued2018
dc.description.abstractInflammation plays a central role in the development of asthma, which is considered an allergic disease with a classic Th2 inflammatory profile. However, cytokine IL-17 has been examined to better understand the pathophysiology of this disease. Severe asthmatic patients experience frequent exacerbations, leading to infection, and subsequently show altered levels of inflammation that are unlikely to be due to the Th2 immune response alone. This study estimates the effects of anti-IL-17 therapy in the pulmonary parenchyma in a murine asthma model exacerbated by LPS. BALB/c mice were sensitized with intraperitoneal ovalbumin and repeatedly exposed to inhalation with ovalbumin, followed by treatment with or without anti-IL-17. Twenty-four hours prior to the end of the 29-day experimental protocol, the two groups received LPS (0.1 mg/ml intratracheal OVA-LPS and OVA-LPS IL-17). We subsequently evaluated bronchoalveolar lavage fluid, performed a lung tissue morphometric analysis, and measured IL-6 gene expression. OVA-LPS-treated animals treated with anti-IL-17 showed decreased pulmonary inflammation, edema, oxidative stress, and extracellular matrix remodeling compared to the non-treated OVA and OVA-LPS groups (p < 0.05). The anti-IL-17 treatment also decreased the numbers of dendritic cells, FOXP3, NF-kappa B, and Rho kinase 1-and 2-positive cells compared to the non-treated OVA and OVA-LPS groups (p < 0.05). In conclusion, these data suggest that inhibition of IL-17 is a promising therapeutic avenue, even in exacerbated asthmatic patients, and significantly contributes to the control of Th1/Th2/Th17 inflammation, chemokine expression, extracellular matrix remodeling, and oxidative stress in a murine experimental asthma model exacerbated by LPS.en
dc.description.affiliationUniv Sao Paulo, Sch Med, Dept Med Sci, Sao Paulo, Brazil|Hosp Sirio Libanes, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Inst Biomed Sci, Dept Biol Sci, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Biol Sci, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Inst Biomed Sci, Dept Biol Sci, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Biol Sci, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt
dc.description.sponsorshipLaboratory of Medical Investigations
dc.description.sponsorshipIDFAPESP: 2013/17944-1
dc.description.sponsorshipIDLaboratory of Medical Investigations: LIM-20 FMUSP
dc.format.extent-
dc.identifierhttps://dx.doi.org/10.3389/fimmu.2017.01835
dc.identifier.citationFrontiers In Immunology. Lausanne, v. 8, p. -, 2018.
dc.identifier.doi10.3389/fimmu.2017.01835
dc.identifier.fileWOS000419371800001.pdf
dc.identifier.issn1664-3224
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54275
dc.identifier.wosWOS:000419371800001
dc.language.isoeng
dc.publisherFrontiers Media Sa
dc.relation.ispartofFrontiers In Immunology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAnti-IL-17en
dc.subjectDistal lungen
dc.subjectAsthmaen
dc.subjectInflammationen
dc.subjectLPS-exacerbateden
dc.titleEffects of Anti-IL-17 on Inflammation, Remodeling, and Oxidative Stress in an Experimental Model of Asthma Exacerbated by LPSen
dc.typeinfo:eu-repo/semantics/article
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